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Mar, 2025;14 (8): e2401834.

Immobilized Multi-Enzyme/Nanozyme Biomimetic Cascade Catalysis for Biosensing Applications.

Xiaoli Cai, Yuteng Huang, Chengzhou Zhu
Author Information
  1. Xiaoli Cai: Academy of Nutrition and Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, P.R. China.
  2. Yuteng Huang: Academy of Nutrition and Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, P.R. China.
  3. Chengzhou Zhu: State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, P.R. China. ORCID
PMID: 38889805 DOI: 10.1002/adhm.202401834

Abstract

Multiple enzyme-induced cascade catalysis has an indispensable role in the process of complex life activities, and is widely used to construct robust biosensors for analyzing various targets. The immobilized multi-enzyme cascade catalysis system is a novel biomimetic catalysis strategy that immobilizes various enzymes with different functions in stable carriers to simulate the synergistic catalysis of multiple enzymes in biological systems, which enables high stability of enzymes and efficiency enzymatic cascade catalysis. Nanozymes, a type of nanomaterial with intrinsic enzyme-like characteristics and excellent stabilities, are also widely applied instead of enzymes to construct immobilized cascade systems, achieving better catalytic performance and reaction stability. Due to good stability, reusability, and remarkably high efficiency, the immobilized multi-enzyme/nanozyme biomimetic cascade catalysis systems show distinct advantages in promoting signal transduction and amplification, thereby attracting vast research interest in biosensing applications. This review focuses on the research progress of the immobilized multi-enzyme/nanozyme biomimetic cascade catalysis systems in recent years. The construction approaches, factors affecting the efficiency, and applications for sensitive biosensing are discussed in detail. Further, their challenges and outlooks for future study are also provided.

Keywords

biomimetic cascade catalysis biosensors enzyme immobilization multi‐enzyme/nanozyme systems nanozymes

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Grants

  1. WJ2023Q015/Hubei Province health and family planning scientific research project
  2. 2023C0301/Wuhan University of Science and Technology
  3. CCNU22JC006/Fundamental Research Funds for the Central Universities
  4. B17019/Program of Introducing Talents of Discipline to Universities of China
  5. 2023AFB122/Natural Science Foundation of Hubei Province

MeSH Term

Biosensing Techniques
Enzymes, Immobilized
Biomimetic Materials
Nanostructures
Catalysis
Biomimetics

Chemicals

Enzymes, Immobilized
Journal Article Review

Word Cloud

Created with Highcharts 10.0.0catalysiscascadesystemsimmobilizedbiomimeticenzymesstabilityefficiencywidelyconstructbiosensorsvarioushighalsomulti-enzyme/nanozymeresearchbiosensingapplicationsMultipleenzyme-inducedindispensableroleprocesscomplexlifeactivitiesusedrobustanalyzingtargetsmulti-enzymesystemnovelstrategyimmobilizesdifferentfunctionsstablecarrierssimulatesynergisticmultiplebiologicalenablesenzymaticNanozymestypenanomaterialintrinsicenzyme-likecharacteristicsexcellentstabilitiesappliedinsteadachievingbettercatalyticperformancereactionDuegoodreusabilityremarkablyshowdistinctadvantagespromotingsignaltransductionamplificationtherebyattractingvastinterestreviewfocusesprogressrecentyearsconstructionapproachesfactorsaffectingsensitivediscusseddetailchallengesoutlooksfuturestudyprovidedImmobilizedMulti-Enzyme/NanozymeBiomimeticCascadeCatalysisBiosensingApplicationsenzymeimmobilizationmulti‐enzyme/nanozymenanozymes

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