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Jun 25, 2024;17 (1): 268.

Hard ticks (Ixodida: Ixodidae) in the Colombian Caribbean harbor the Jingmen tick virus: an emerging arbovirus of public health concern.

Yesica López, Richard Thomas, Sebastián Muñoz-Leal, Yeimi López-Mejia, Ketty Galeano, Alejandra Garcia, Luis Romero, Daniel Echeverri-De la Hoz, Caty Martinez, Alfonso Calderón, Bertha Gastelbondo, Héctor Contreras, Gino Olivieri, Luis Rubiano, Luis Paternina, Richard Hoyos-López, Anggie Ortiz, Evelyn Garay, Maira Alemán-Santos, Ricardo Rivero, Jorge Miranda, Luis Florez, Jolaime Ballesteros, Verónica Contreras, Vaneza Tique, Pedro Fragoso, Camilo Guzman, German Arrieta, Salim Mattar
Author Information
  1. Yesica López: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  2. Richard Thomas: Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile.
  3. Sebastián Muñoz-Leal: Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile.
  4. Yeimi López-Mejia: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  5. Ketty Galeano: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  6. Alejandra Garcia: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  7. Luis Romero: Universidad de Sucre, Investigaciones Biomédicas, Sucre, Colombia.
  8. Daniel Echeverri-De la Hoz: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  9. Caty Martinez: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  10. Alfonso Calderón: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  11. Bertha Gastelbondo: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  12. Héctor Contreras: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  13. Gino Olivieri: Grupo de Investigación Parasitología y Agroecología Milenio, Universidad Popular del Cesar, Valledupar Cesar, Colombia.
  14. Luis Rubiano: Grupo de Investigación Parasitología y Agroecología Milenio, Universidad Popular del Cesar, Valledupar Cesar, Colombia.
  15. Luis Paternina: Universidad de Sucre, Investigaciones Biomédicas, Sucre, Colombia.
  16. Richard Hoyos-López: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  17. Anggie Ortiz: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  18. Evelyn Garay: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  19. Maira Alemán-Santos: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  20. Ricardo Rivero: Paul G. Allen School for Global Health, Washington State University, Pullman, WA, USA.
  21. Jorge Miranda: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  22. Luis Florez: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  23. Jolaime Ballesteros: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  24. Verónica Contreras: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  25. Vaneza Tique: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  26. Pedro Fragoso: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  27. Camilo Guzman: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  28. German Arrieta: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia.
  29. Salim Mattar: Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Córdoba, Colombia. smattar@correo.unicordoba.edu.co.
PMID: 38918818 DOI: 10.1186/s13071-024-06362-x

Abstract

BACKGROUND: Ticks are obligate hematophagous ectoparasites involved in transmitting viruses of public health importance. The objective of this work was to identify the Jingmen tick virus in hard ticks from the Colombian Caribbean, an arbovirus of importance for public health.
METHODS: Ticks were collected in rural areas of Córdoba and Cesar, Colombia. Taxonomic identification of ticks was carried out, and pools of 13 individuals were formed. RNA extraction was performed. Library preparation was performed with the MGIEasy kit, and next-generation sequencing (NGS) with MGI equipment. Bioinformatic analyses and taxonomic assignments were performed using the Galaxy platform, and phylogenetic analyses were done using IQ-TREE2.
RESULTS: A total of 766 ticks were collected, of which 87.33% (669/766) were Rhipicephalus microplus, 5.4% (42/766) Dermacentor nitens, 4.2% (32/766) Rhipicephalus linnaei, and 3.0% (23/766) Amblyomma dissimile. Complete and partial segments 1, 2, 3, and 4 of Jingmen tick virus (JMTV) were detected in the metatranscriptome of the species R. microplus, D. nitens, and A. dissimile. The JMTVs detected are phylogenetically related to JMTVs detected in Aedes albopictus in France, JMTVs detected in R. microplus in Trinidad and Tobago, JMTVs in R. microplus and A. variegatum in the French Antilles, and JMTVs detected in R. microplus in Colombia. Interestingly, our sequences clustered closely with JMTV detected in humans from Kosovo.
CONCLUSIONS: JMTV was detected in R. microplus, D. nitens, and A. dissimile. JMTV could pose a risk to humans. Therefore, it is vital to establish epidemiological surveillance measures to better understand the possible role of JMTV in tropical diseases.

Keywords

Amblyomma dissimile Dermacentor nitens Rhipicephalus microplus Jingmen tick virus Next-generation sequencing Tick-borne disease Virus

References

  1. Proc Natl Acad Sci U S A. 2014 May 6;111(18):6744-9 [PMID: 24753611]
  2. Bioinformatics. 2018 Sep 1;34(17):i884-i890 [PMID: 30423086]
  3. Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296 [PMID: 33885785]
  4. Viruses. 2021 Dec 19;13(12): [PMID: 34960816]
  5. Nat Methods. 2012 Mar 04;9(4):357-9 [PMID: 22388286]
  6. Cell Host Microbe. 2016 Sep 14;20(3):357-367 [PMID: 27569558]
  7. Viruses. 2019 Nov 17;11(11): [PMID: 31744216]
  8. Infect Genet Evol. 2018 Nov;65:6-11 [PMID: 30006045]
  9. Cancer Res. 2017 Nov 1;77(21):e23-e26 [PMID: 29092932]
  10. Front Microbiol. 2022 Jul 01;13:932224 [PMID: 35847110]
  11. Nucleic Acids Res. 2002 Jul 15;30(14):3059-66 [PMID: 12136088]
  12. J Virol. 2015 Oct 21;90(2):659-69 [PMID: 26491167]
  13. J Mol Evol. 1981;17(6):368-76 [PMID: 7288891]
  14. mSphere. 2023 Oct 24;8(5):e0028123 [PMID: 37702505]
  15. Bioinformatics. 2015 May 15;31(10):1674-6 [PMID: 25609793]
  16. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6 [PMID: 14681350]
  17. J Gen Virol. 2017 Jan;98(1):2-3 [PMID: 28218572]
  18. Transbound Emerg Dis. 2022 May;69(3):1387-1403 [PMID: 33840161]
  19. Genomics. 2023 Nov;115(6):110734 [PMID: 37890641]
  20. EBioMedicine. 2019 May;43:317-324 [PMID: 31003930]
  21. Ticks Tick Borne Dis. 2020 Sep;11(5):101426 [PMID: 32473925]
  22. Microorganisms. 2021 Aug 03;9(8): [PMID: 34442732]
  23. Ticks Tick Borne Dis. 2019 Oct;10(6):101252 [PMID: 31255534]
  24. Sci Rep. 2019 Jul 18;9(1):10421 [PMID: 31320705]
  25. Sci Rep. 2018 Nov 5;8(1):16315 [PMID: 30397237]
  26. Infect Genet Evol. 2020 Oct;84:104411 [PMID: 32531517]
  27. J Med Microbiol. 2020 Jun;69(6):781-791 [PMID: 32478654]
  28. Front Cell Infect Microbiol. 2017 Jul 11;7:298 [PMID: 28744449]
  29. Infect Genet Evol. 2021 Dec;96:105103 [PMID: 34619391]
  30. Mol Biol Evol. 2015 Jan;32(1):268-74 [PMID: 25371430]
  31. mSphere. 2019 Nov 6;4(6): [PMID: 31694898]
  32. Nat Methods. 2017 Jun;14(6):587-589 [PMID: 28481363]
  33. Sci Rep. 2023 Nov 14;13(1):19824 [PMID: 37963929]
  34. N Engl J Med. 2019 May 30;380(22):2116-2125 [PMID: 31141633]
  35. Structure. 2016 Dec 6;24(12):2041-2042 [PMID: 27926832]
  36. J Mol Biol. 1990 Oct 5;215(3):403-10 [PMID: 2231712]
  37. Syst Biol. 2010 May;59(3):307-21 [PMID: 20525638]

MeSH Term

Animals
Colombia
Phylogeny
Ixodidae
Arboviruses
Caribbean Region
Female
Male
Public Health
High-Throughput Nucleotide Sequencing
Rhipicephalus
Humans
Amblyomma
Dermacentor
Journal Article
Article has an altmetric score of 3

Word Cloud

Created with Highcharts 10.0.0detectedJMTVJMTVsJingmentickticksmicroplusnitensdissimilein RpublichealthvirusperformedTicksimportanceColombianCaribbeanarboviruscollectedColombiasequencinganalysesusing43microplus inhumansBACKGROUND:obligatehematophagousectoparasitesinvolvedtransmittingviruses TheobjectiveworkidentifyhardMETHODS:ruralareasCórdobaCesarTaxonomicidentificationcarriedpools13individualsformedRNAextractionLibrarypreparationMGIEasykitnext-generationNGSMGIequipmentBioinformatictaxonomicassignmentsGalaxyplatformphylogeneticdoneIQ-TREE2RESULTS:total7668733%669/766were Rhipicephalus54%42/766 Dermacentor2%32/766 Rhipicephaluslinnaei0%23/766 AmblyommaCompletepartialsegments12metatranscriptomespeciesRD and Aphylogeneticallyrelatedin Aedesalbopictus inFranceTrinidadTobagomicroplus and Avariegatum inFrenchAntillesInterestinglysequencesclusteredcloselyKosovoCONCLUSIONS: Dand AposeriskThereforevitalestablishepidemiologicalsurveillancemeasuresbetterunderstandpossibleroletropicaldiseasesHardIxodida:Ixodidaeharborvirus:emergingconcernAmblyommaDermacentorRhipicephalusNext-generationTick-bornediseaseVirus

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