Miniaturizable Chemiluminescence System for ATP Detection in Water.
Giuseppe E Capuano, Domenico Corso, Roberta Farina, Gianni Pezzotti Escobar, Giuseppe A Screpis, Maria Anna Coniglio, Sebania Libertino
Author Information
Giuseppe E Capuano: Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy. ORCID
Domenico Corso: Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy.
Roberta Farina: Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy. ORCID
Gianni Pezzotti Escobar: URT "LabSens of Beyond Nano" of the Department of Physical Sciences and Technologies of Matter, National Research Council (CNR-DSFTM-ME), Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy. ORCID
Giuseppe A Screpis: Department of Medical, Surgical Sciences and Advanced Technologies, University of Catania, Via S. Sofia 78, 95123 Catania, Italy.
Maria Anna Coniglio: Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy. ORCID
Sebania Libertino: Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy. ORCID
We present the design, fabrication, and testing of a low-cost, miniaturized detection system that utilizes chemiluminescence to measure the presence of adenosine triphosphate (ATP), the energy unit in biological systems, in water samples. The ATP-luciferin chemiluminescent solution was faced to a silicon photomultiplier (SiPM) for highly sensitive real-time detection. This system can detect ATP concentrations as low as 0.2 nM, with a sensitivity of 79.5 A/M. Additionally, it offers rapid response times and can measure the characteristic time required for reactant diffusion and mixing within the reaction volume, determined to be 0.3 �� 0.1 s. This corresponds to a diffusion velocity of approximately 44 �� 14 mm/s.
