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Regenerative medicine
06 02, 2024;19 (6): 327-343.

PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats.

Derya Burcu Hazer Rosberg, Lena Stenberg, Margit Mahlapuu, Lars B Dahlin
Author Information
  1. Derya Burcu Hazer Rosberg: Department of Translational Medicine - Hand Surgery, Lund University, Skåne University Hospital, SE-20502, Malmö, Sweden. ORCID
  2. Lena Stenberg: Department of Translational Medicine - Hand Surgery, Lund University, Skåne University Hospital, SE-20502, Malmö, Sweden. ORCID
  3. Margit Mahlapuu: Department of Chemistry & Molecular Biology, Göteborg University , SE-40530, Göteborg, Sweden. ORCID
  4. Lars B Dahlin: Department of Translational Medicine - Hand Surgery, Lund University, Skåne University Hospital, SE-20502, Malmö, Sweden. ORCID
PMID: 38957920 DOI: 10.1080/17460751.2024.2361515

Abstract

Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats. PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed. Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups. PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages.

Keywords

HSP27 PXL01 Schwann cells apoptosis inflammation macrophages nerve injury nerve repair sensory neuron sodium hyaluronate

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Grants

  1. /Skånes universitetssjukhus
  2. /Elly Ollson's Foundation
  3. 2018-0104/Region Skåne
  4. DIA2020-492/Sydvästra Skånes Diabetesförening and the Swedish Diabetes Foundation
  5. /Lund University Medical Faculty Foundation

MeSH Term

Animals
Schwann Cells
Macrophages
Nerve Regeneration
Rats, Wistar
Sciatic Nerve
Axons
Rats
Ganglia, Spinal
Male
Journal Article

Word Cloud

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