Impact of Photobiomodulation on IL1β and TGFβ-1 concentrations in patients with aphthous stomatitis.

Dalia Saleem Kareem, Layla M H Al-Ameri, Ammar Saleh Alalawi
Author Information
  1. Dalia Saleem Kareem: Medical and Biological applications Branch Institute of Laser for Postgraduate Studies, University of Baghdad, Baghdad, Iraq. Dalia.ugla2102m@ilps.uobaghdad.edu.iq.
  2. Layla M H Al-Ameri: Medical and Biological applications Branch Institute of Laser for Postgraduate Studies, University of Baghdad, Baghdad, Iraq.
  3. Ammar Saleh Alalawi: Laser/Dentistry (oral surgery), Al-Emamein Al-Kadhimein Madical City, Baghdad, Iraq.

Abstract

Laser therapy has shown effectiveness in promoting wound healing by influencing various physiological factors such as blood flow, cytokines, histamine, nerve signals, lymphocyte function, tissue oxygenation, and cell growth. This study aims to evaluate the therapeutic efficacy of Photobiomodulation (PBM) treatment, by using diode laser, in modifying the levels of interleukin-1 beta (IL1β) and transforming growth factor beta-1 (TGFβ-1) in patients diagnosed with aphthous stomatitis. A before-after interventional design was conducted over 10 months with 20 subjects. Data on demographic details and serum concentrations of IL1β and TGFβ-1 were collected pre-treatment and on Days 3 and 7 post-treatments. The intervention involved a single session of four 30-second applications of a QuickLase dual-wavelength laser operating at 980 nm. Results show significant reductions in IL1β and TGFβ-1 levels after 7 days of treatment, indicating a time-dependent effect of PBM therapy on these inflammatory markers. The findings suggest that PBM therapy holds promise as an intervention for reducing inflammation associated with aphthous stomatitis.

Keywords

References

  1. Porter SR, Hegarty A, Kaliakatsou F, Hodgson TA, Scully C (2000) Recurrent aphthous stomatitis. Clin Dermatol 18(5):569–578 [PMID: 11134852]
  2. Sharquie KE, Al-Tammimy SM, Al-Mashhadani S, Hayani RK, Al-Nuaimy AA (2006) Lactic acid 5% mouthwash is an effective mode of therapy in treatment of recurrent aphthous ulcerations. Dermatol Online J. ;12(7)
  3. Rivera C, Muñoz-Pastén M, Núñez-Muñoz E, Hernández-Olivos R (2022) Recurrent aphthous stomatitis affects quality of life. A case-control study. Clinical, cosmetic and investigational dentistry. 1:217–223
  4. Akintoye SO, Greenberg MS (2014) Recurrent aphthous stomatitis. Dent Clin 58(2):281–297
  5. Pestka S, Krause CD, Sarkar D, Walter MR, Shi Y, Fisher PB (2004) Interleukin-10 and related cytokines and receptors. Annu Rev Immunol 22:929–979 [PMID: 15032600]
  6. Turner D, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV (1997) An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24(1):1–8 [PMID: 9043871]
  7. Sabat R (2010) IL-10 family of cytokines. Cytokine Growth Factor Rev 21(5):315–324 [PMID: 21112807]
  8. Kang J, Lee MS, Copland JA III, Luxon BA, Gorenstein DG (2008) Combinatorial selection of a single stranded DNA thioaptamer targeting TGF-β1 protein. Bioorg Med Chem Lett 18(6):1835–1839 [PMID: 18294846]
  9. Field EA, Allan RB (2003) Oral ulceration–aetiopathogenesis, clinical diagnosis and management in the gastrointestinal clinic. Aliment Pharmacol Ther 18(10):949 [PMID: 14616160]
  10. Mohammed SE, Al-ameri LM (2021) Laser Biostimulation Effect on Human sperm motility. Iraqi J Laser. ;20(1)
  11. Al-ameri LM, Faris RA (2020) Biochemical immune effects of low power laser irradiation on leukemia and breast cancer: a review. EurAsian J Biosci. ;14(2)
  12. Jawad HA, Hamdi SA (2015) Non Anesthetic Second Stage Implant surgery by 970nm Diode laser. IOSR J Dent Med Sci Ver I 14(6):2279–2861
  13. Al-Karadaghi TS, Gutknecht N, Jawad HA, Vanweersch L, Franzen R (2015) Evaluation of temperature elevation during root canal treatment with dual wavelength laser: 2780 nm er, Cr: YSGG and 940 nm diode. Photomed Laser Surg 33(9):460–466 [PMID: 26332917]
  14. Al-Karadaghi TS, Franzen R, Jawad HA, Gutknecht N (2015) Investigations of radicular dentin permeability and ultrastructural changes after irradiation with Er, Cr: YSGG laser and dual wavelength (2780 and 940 nm) laser. Lasers Med Sci 30:2115–2121 [PMID: 25935585]
  15. Ad’hiah AH, Al-Ameri LM, Maki AM, Wang Q, ALQaisi MH (2015) Modulating heat shock proteins 70 and 90 expression by low power laser irradiation (635nm and 780nm) in Jurkat E6. 1 T-lymphocyte leukemia cell line. J Lasers Med Sci 6(1):17 [PMID: 25699163]
  16. Al-Alawi AS, Jawad HA, Ismael AM (2020) Clinical evaluation of low level laser therapy in skin wound healing in maxillofacial surgery. Iraqi J Med Sci 20(4):68
  17. Owaid NA, Jawad HA, Maazil AA (2016) Evaluation of low level laser therapy using 785 nm Diode Laser on the enhancement of Chronic Wound Healing. Iraqi J Laser 15(B):41–46
  18. Pereira LB, Chimello DT, Wimmers Ferreira MR, Bachmann L, Rosa AL, Bombonato-Prado KF (2012) Low-level laser therapy influences mouse odontoblast-like cell response in vitro. Photomed Laser Surg 30(4):206–213 [PMID: 22375953]
  19. Ślebioda Z, Dorocka-Bobkowska B (2020) Low-level laser therapy in the treatment of recurrent aphthous stomatitis and oral lichen planus: a literature review. Adv Dermatology Allergology/Postępy Dermatologii i Alergologii 37(4):475–481
  20. Safavi SM, Kazemi B, Esmaeili M, Fallah A, Modarresi A, Mir M (2008) Effects of low-level He–Ne laser irradiation on the gene expression of IL-1β, TNF-α, IFN-γ, TGF-β, bFGF, and PDGF in rat’s gingiva. Lasers Med Sci 23:331–335 [PMID: 17786499]
  21. Mizutani K, Musya Y, Wakae K, Kobayashi T, Tobe M, Taira K, Harada T (2004) A clinical study on serum prostaglandin E2 with low-level laser therapy. Photomed Laser Therapy 22(6):537–539
  22. Vale FA, Moreira MS, de Almeida FC, Ramalho KM (2015) Low-level laser therapy in the treatment of recurrent aphthous ulcers: a systematic review. Sci World J. ;2015
  23. Al-Alawe AS, Jawad HA Efficacy of low level diode laser in skin wound healing (experimental study). Scientific Journal Published by the College of Dentistry–University of Baghdad.:70.
  24. Al-Wattar WM, Abdulluh BH, Mahmmod AS (2013) Irradiation effect of 780-805nm diode laser on wound healing in mice. J Bagh Coll Dentistry 25(1):48–52
  25. Oton-Leite AF, Silva GB, Morais MO, Silva TA, Leles CR, Valadares MC, Pinezi JC, Batista AC, Mendonça EF (2015) Effect of low‐level laser therapy on chemoradiotherapy‐induced oral mucositis and salivary inflammatory mediators in head and neck cancer patients. Lasers Surg Med 47(4):296–305 [PMID: 25824475]
  26. Balakrishnan A, Arunachalam LT, Sudhakar U (2022) Evaluation of TGF-β1 in gingival crevicular fluid and clinical parameters of smoker and nonsmoker patients treated with low-level laser therapy as an adjunct to scaling and root planing. Lasers Dent Sci 6(1):55–61
  27. Gavish L, Perez L, Gertz SD (2006) Low-level laser irradiation modulates matrix metalloproteinase activity and gene expression in porcine aortic smooth muscle cells. Lasers Surg Medicine: Official J Am Soc Laser Med Surg 38(8):779–786
  28. Mesquita-Ferrari RA, Martins MD, Silva JA, Da Silva TD, Piovesan RF, Pavesi VC, Bussadori SK, Fernandes KP (2011) Effects of low-level laser therapy on expression of TNF-α and TGF-β in skeletal muscle during the repair process. Lasers Med Sci 26:335–340 [PMID: 21053039]
  29. Singer AJ, Clark RA (1999) Cutaneous wound healing. N Engl J Med 341(10):738–746 [PMID: 10471461]
  30. Morikawa M, Derynck R, Miyazono K (2016) TGF-β and the TGF-β family: context-dependent roles in cell and tissue physiology. Cold Spring Harb Perspect Biol 8(5):a021873 [PMID: 27141051]
  31. Massagué J (2012) TGFβ signalling in context. Nat Rev Mol Cell Biol 13(10):616–630 [PMID: 22992590]
  32. Murphy-Ullrich JE, Poczatek M (2000) Activation of latent TGF-β by thrombospondin-1: mechanisms and physiology. Cytokine Growth Factor Rev 11(1–2):59–69 [PMID: 10708953]
  33. Belli M, Fernandes CR, Neves LM, Mourão V, Barbieri R, Esquisatto MA, Amaral ME, Santos GM, Mendonça FA (2014) Application of 670 nm InGaP laser and microcurrent favors the healing of second-degree burns in Wistar rats. Laser Phys 25(2):025602
  34. Jácomo AC, de Andrade Velozo K, Lotti RG, Neves LM, de Gaspari de Gaspi FO, Esquisatto MA, do Amaral ME, Mendonça FA, dos Santos GM (2015) Activity of Porophyllum ruderale leaf extract and 670-nm InGaP laser during burns repair in rats. BMC Complement Altern Med 15:1–9
  35. Maligieri LA, Neves LM, de Morais DT, Domingues RF, de Aro AA, Pimentel ER, do Amaral ME, Esquisatto MA, Dos Santos GM, Mendonça FA (2017) Differing energy densities with laser 670 nm InGaP controls inflammation and collagen reorganization in burns. Burns 43(7):1524–1531 [PMID: 28778761]
  36. Alves N, Zaror C, Sol MD, Bagnato VS, Deana NF (2022) Effects of laser photobiomodulation on TGF-ß and VEGF expression in burn wound: systematic review and meta-analysis in the animal model. Int J Morphology 40(1):194–203
  37. Türkuçar S, Bülbül G, Ünsal E, Özer E, Erdağ TK, Erkoç E, Makay B (2023) Exploring the immunological basis of periodic fever, aphthous stomatitis, pharyngitis, and adenitis (PFAPA) syndrome: immunohistochemical staining features of palatine tonsils. Clin Rheumatol 42(7):1911–1916 [PMID: 36892710]
  38. Chiang CP, Chang JY, Wang YP, Wu YH, Wu YC, Sun A (2019) Recurrent aphthous stomatitis–Etiology, serum autoantibodies, anemia, hematinic deficiencies, and management. J Formos Med Assoc 118(9):1279–1289 [PMID: 30446298]

MeSH Term

Humans
Interleukin-1beta
Low-Level Light Therapy
Adult
Female
Male
Transforming Growth Factor beta1
Stomatitis, Aphthous
Lasers, Semiconductor
Middle Aged
Young Adult

Chemicals

Interleukin-1beta
Transforming Growth Factor beta1
IL1B protein, human
TGFB1 protein, human

Word Cloud

Created with Highcharts 10.0.0IL1βTGFβ-1therapyPhotobiomodulationPBMaphthousstomatitisgrowthtreatmentlaserlevelspatientsconcentrations7interventionLasershowneffectivenesspromotingwoundhealinginfluencingvariousphysiologicalfactorsbloodflowcytokineshistaminenervesignalslymphocytefunctiontissueoxygenationcellstudyaimsevaluatetherapeuticefficacyusingdiodemodifyinginterleukin-1betatransformingfactorbeta-1diagnosedbefore-afterinterventionaldesignconducted10months20subjectsDatademographicdetailsserumcollectedpre-treatmentDays3post-treatmentsinvolvedsinglesessionfour30-secondapplicationsQuickLasedual-wavelengthoperating980 nmResultsshowsignificantreductionsdaysindicatingtime-dependenteffectinflammatorymarkersfindingssuggestholdspromisereducinginflammationassociatedImpactAphthousulcer

Similar Articles

Cited By