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Environmental science and pollution research international
Aug, 2024;31 (36): 49244-49254.

Winery wastewater treatment by microalgae Chlorella sorokiniana and characterization of the produced biomass for value-added products.

Eirini Zkeri, Maria Mastori, Argyri Xenaki, Evangelia Kritikou, Marios Kostakis, Marilena Dasenaki, Niki Maragou, Michail S Fountoulakis, Nikolaos S Thomaidis, Athanasios S Stasinakis
Author Information
  1. Eirini Zkeri: Department of Environment, University of the Aegean, 81100, Mytilene, Greece.
  2. Maria Mastori: Department of Environment, University of the Aegean, 81100, Mytilene, Greece.
  3. Argyri Xenaki: Department of Environment, University of the Aegean, 81100, Mytilene, Greece.
  4. Evangelia Kritikou: Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771, Athens, Greece.
  5. Marios Kostakis: Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771, Athens, Greece.
  6. Marilena Dasenaki: Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771, Athens, Greece.
  7. Niki Maragou: Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771, Athens, Greece.
  8. Michail S Fountoulakis: Department of Environment, University of the Aegean, 81100, Mytilene, Greece.
  9. Nikolaos S Thomaidis: Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771, Athens, Greece.
  10. Athanasios S Stasinakis: Department of Environment, University of the Aegean, 81100, Mytilene, Greece. astas@env.aegean.gr. ORCID
PMID: 39060890 DOI: 10.1007/s11356-024-34446-9

Abstract

The microalgae Chlorella sorokiniana was used for the treatment of winery wastewater (WWW). Batch experiments were initially conducted to investigate how biomass acclimatization in different media, dilution of wastewater, and addition of ammonium nitrogen (NH-N) affect the growth of microalgae and the removal of major pollutants. Afterwards, two sequencing batch reactor (SBR) systems were tested applying different configurations and hydraulic retention times. The biomass collected at the end of the experiments was characterized for proteins, lipids, carbohydrates, amino acid profile, and the existence of lutein, ��-carotene, chlorophyll a, and tocopherols. Batch experiments showed that Chlorella sorokiniana acclimatization to urban wastewater enhanced the removal of NH-N and total phosphorus (TP). The operation of a two-stage SBR system achieved COD and NH-N removal equal to 85��������9% and 91��������20%, respectively, while the use of a single-stage system feeding with anaerobically pretreated WWW resulted to COD and NH-N removal of 78��������9% and 95��������9%, respectively. Analyses of biomass showed higher protein content (up to 58.8%) in batch experiments with NH-N addition as well as in SBR experiments. The cultivation of microalgae under SBR conditions enhanced the production of pigments and tocopherols. The maximum concentrations of 1075 mg kg, 45.5 mg kg, and 131.2 mg kg were achieved for lutein, ��-carotene, and tocopherols, respectively, in the one-stage system. Our findings suggested that Chlorella sorokiniana cultivation in WWW not only removed nutrients from WWW but also could potentially serve for the production of value-added ingredients used in food industry, cosmetics, and animal feedstock.

Keywords

Carotenoids Circular economy Cultivation Microalgal technology Valorization Wine production wastewater

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Grants

  1. MIS 5046750/European Regional Development Fund

MeSH Term

Chlorella
Wastewater
Microalgae
Biomass
Waste Disposal, Fluid
Nitrogen

Chemicals

Wastewater
Nitrogen
Journal Article
Article has an altmetric score of 2

Word Cloud

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