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Antibiotics (Basel, Switzerland)
Jun 21, 2024;13 (7):

Modification and Synergistic Studies of a Novel Frog Antimicrobial Peptide against Biofilms.

Xinze Liu, Daning Shi, Shiya Cheng, Xiaoling Chen, Chengbang Ma, Yangyang Jiang, Tao Wang, Tianbao Chen, Chris Shaw, Lei Wang, Mei Zhou
Author Information
  1. Xinze Liu: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
  2. Daning Shi: Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 100081, China.
  3. Shiya Cheng: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
  4. Xiaoling Chen: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
  5. Chengbang Ma: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
  6. Yangyang Jiang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
  7. Tao Wang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. ORCID
  8. Tianbao Chen: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. ORCID
  9. Chris Shaw: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
  10. Lei Wang: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. ORCID
  11. Mei Zhou: Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. ORCID
PMID: 39061256 DOI: 10.3390/antibiotics13070574

Abstract

The overuse of traditional antibiotics has resulted in bacterial resistance and seriously compromised the therapeutic efficacy of traditional antibiotics, making the exploration of new antimicrobials particularly important. Several studies have shown that bioactive peptides have become an important source of new antimicrobial drugs due to their broad-spectrum antibacterial action and lack of susceptibility to resistance. In this study, a novel bioactive peptide Nigrosin-6VL was characterised from the skin secretion of the golden cross band frog, , by using the 'shotgun' cloning strategy. Modifications on the Rana Box of Nigrosin-6VL revealed its critical role in antimicrobial functions. The peptide analogue, 2170-2R, designed to preserve the Rana Box structure while enhancing cationicity, exhibited improved therapeutic efficacy, particularly against Gram-negative bacteria, with a therapeutic value of 45.27. Synergistic studies demonstrated that 2170-2R inherits the synergistic antimicrobial activities of the parent peptides and effectively enhances the antimicrobial capacity of cefepime and gentamicin against both planktonic cells and biofilms. Specifically, 2170-2R can synergise effectively with cefepime and gentamicin against different strains of biofilms. Consequently, 2170-2R holds promise as a potent antimicrobial agent developed to combat infections induced by .

Keywords

Pseudomonas aeruginosa antibiotics antimicrobial peptides (AMPs) co-administration peptide modification

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Journal Article

Word Cloud

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