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Frontiers in microbiology
2024;15 1439373.

Genomic study of strains co-harboring and reveals a large multidrug-resistant plasmid encoding these carbapenemases in Brazil.

Daiana Cristina Silva Rodrigues, Melise Chaves Silveira, Bruno Rocha Pribul, Bruna Ribeiro Sued Karam, Renata Cristina Pic��o, Gabriela Bergiante Kraychete, Felicidade Mota Pereira, Rildo Mendes de Lima, Antonio Kleber Gomes de Souza, Robson Souza Le��o, Elizabeth Andrade Marques, Cl��udio Marcos Rocha-de-Souza, Ana Paula D'Alincourt Carvalho-Assef
Author Information
  1. Daiana Cristina Silva Rodrigues: Laborat��rio de Bacteriologia Aplicada �� Sa��de ��nica e Resist��ncia Antimicrobiana (LabSUR), Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
  2. Melise Chaves Silveira: Laborat��rio de Bacteriologia Aplicada �� Sa��de ��nica e Resist��ncia Antimicrobiana (LabSUR), Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
  3. Bruno Rocha Pribul: Laborat��rio de Bacteriologia Aplicada �� Sa��de ��nica e Resist��ncia Antimicrobiana (LabSUR), Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
  4. Bruna Ribeiro Sued Karam: Laborat��rio de Bacteriologia Aplicada �� Sa��de ��nica e Resist��ncia Antimicrobiana (LabSUR), Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
  5. Renata Cristina Pic��o: Laborat��rio de Investiga����o em Microbiologia M��dica (LIMM), Instituto de Microbiologia Paulo de G��es (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  6. Gabriela Bergiante Kraychete: Laborat��rio de Investiga����o em Microbiologia M��dica (LIMM), Instituto de Microbiologia Paulo de G��es (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
  7. Felicidade Mota Pereira: Laborat��rio Central de Sa��de P��blica do Estado da Bahia (LACEN-BA), Bahia, Brazil.
  8. Rildo Mendes de Lima: Laborat��rio Central de Sa��de P��blica da Funda����o de Vigil��ncia em Sa��de do Amazonas (LACEN-AM/FVS-RCP), Amazonas, Brazil.
  9. Antonio Kleber Gomes de Souza: Laborat��rio Reunidos Ltda., Amazonas, Brazil.
  10. Robson Souza Le��o: Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Faculdade de Ci��ncias M��dicas (FCM), Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brazil.
  11. Elizabeth Andrade Marques: Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Faculdade de Ci��ncias M��dicas (FCM), Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brazil.
  12. Cl��udio Marcos Rocha-de-Souza: Laborat��rio de Bacteriologia Aplicada �� Sa��de ��nica e Resist��ncia Antimicrobiana (LabSUR), Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
  13. Ana Paula D'Alincourt Carvalho-Assef: Laborat��rio de Bacteriologia Aplicada �� Sa��de ��nica e Resist��ncia Antimicrobiana (LabSUR), Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
PMID: 39086650 DOI: 10.3389/fmicb.2024.1439373

Abstract

Introduction: contributes significantly to the global issue of multidrug-resistant (MDR) nosocomial infections. Often, these strains demonstrate resistance to carbapenems (MDR-CRAB), the first-line treatment for infections instigated by MDR . Our study focused on the antimicrobial susceptibility and genomic sequences related to plasmids from 12 clinical isolates of that carry both the blaOXA-58 and carbapenemase genes.
Methods: Whole-genome sequencing with long-read technology was employed for the characterization of an A. baumannii plasmid that harbors the and blaNDM-1 genes. The location of the and genes was confirmed through Southern blot hybridization assays. Antimicrobial susceptibility tests were conducted, and molecular characterization was performed using PCR and PFGE.
Results: Multilocus Sequence Typing analysis revealed considerable genetic diversity among and positive strains in Brazil. It was confirmed that these genes were located on a plasmid larger than 300 kb in isolates from the same hospital, which also carry other antimicrobial resistance genes. Different genetic contexts were observed for the co-occurrence of these carbapenemase-encoding genes in Brazilian strains.
Discussion: The propagation of and genes on the same plasmid, which also carries other resistance determinants, could potentially lead to the emergence of bacterial strains resistant to multiple classes of antimicrobials. Therefore, the characterization of these strains is of paramount importance for monitoring resistance evolution, curbing their rapid global dissemination, averting outbreaks, and optimizing therapy.

Keywords

Acinetobacter baumannii NDM-1 carbapenemase OXA-58 carbapenemase co-harboring genomic plasmids

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Journal Article

Word Cloud

Created with Highcharts 10.0.0genesstrainsresistanceplasmidcarbapenemasecharacterizationglobalmultidrug-resistantMDRinfectionsstudyantimicrobialsusceptibilitygenomicplasmidsisolatescarrybaumanniiconfirmedgeneticBrazilalsoco-harboringIntroduction:contributessignificantlyissuenosocomialOftendemonstratecarbapenemsMDR-CRABfirst-linetreatmentinstigatedfocusedsequencesrelated12clinicalblaOXA-58Methods:Whole-genomesequencinglong-readtechnologyemployedharborsblaNDM-1locationSouthernblothybridizationassaysAntimicrobialtestsconductedmolecularperformedusingPCRPFGEResults:MultilocusSequenceTypinganalysisrevealedconsiderablediversityamongpositivelocatedlarger300kbhospitalDifferentcontextsobservedco-occurrencecarbapenemase-encodingBrazilianDiscussion:propagationcarriesdeterminantspotentiallyleademergencebacterialresistantmultipleclassesantimicrobialsThereforeparamountimportancemonitoringevolutioncurbingrapiddisseminationavertingoutbreaksoptimizingtherapyGenomicrevealslargeencodingcarbapenemasesAcinetobacterNDM-1OXA-58

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