OpenLB
Open Library of Bioscience
Advanced Search
PloS one
2024;19 (8): e0308334.

Long-term exposure to polychlorinated biphenyl 126 induces liver fibrosis and upregulates miR-155 and miR-34a in C57BL/6 mice.

Fernanda Torres Quitete, Ananda Vitória Silva Teixeira, Thamara Cherem Peixoto, Bruna Cadete Martins, Geórgia Correa Atella, Angela de Castro Resende, Daniela de Barros Mucci, Fabiane Martins, Julio Beltrame Daleprane
Author Information
  1. Fernanda Torres Quitete: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
  2. Ananda Vitória Silva Teixeira: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
  3. Thamara Cherem Peixoto: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
  4. Bruna Cadete Martins: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
  5. Geórgia Correa Atella: Medical Biochemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  6. Angela de Castro Resende: Department of Pharmacology, Rio de Janeiro State University, Rio de Janeiro, Brazil.
  7. Daniela de Barros Mucci: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil. ORCID
  8. Fabiane Martins: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
  9. Julio Beltrame Daleprane: Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil. ORCID
PMID: 39133714 DOI: 10.1371/journal.pone.0308334

Abstract

Environmental pollutants, including polychlorinated biphenyls (PCBs), act as endocrine disruptors and impair various physiological processes. PCB 126 is associated with steatohepatitis, fibrosis, cirrhosis, and other hepatic injuries. These disorders can be regulated by microRNAs (miRNAs). Therefore, this study aimed to investigate the role of miRNAs in non-alcoholic fatty liver disease associated with exposure to PCB 126. Adult male C57BL/6 mice were exposed to PCB 126 (5 μmol/kg of body weight) for 10 weeks. The PCB group showed lipid accumulation in the liver in the presence of macro- and microvesicular steatosis and fibrosis with increased inflammatory and profibrotic gene expression, consistent with non-alcoholic steatohepatitis (NASH). PCB exposure also upregulated miR-155 and miR-34a, which induce the expression of proinflammatory cytokines and inflammation in the liver and reduce the expression of peroxisome proliferator-activated receptor α, which, in turn, impairs lipid oxidation and hepatic steatosis. Therefore, the present study showed that PCB 126 induced NASH via potential mechanisms involving miR-155 and miR-34a, which may contribute to the development of new diagnostic markers and therapeutic strategies.

References

  1. Cell Metab. 2009 Apr;9(4):327-38 [PMID: 19356714]
  2. Biomed Pharmacother. 2021 Dec;144:112314 [PMID: 34634561]
  3. Sci Total Environ. 2020 Jun 10;720:137597 [PMID: 32143051]
  4. J Diabetes Metab Disord. 2015 Dec 10;14:88 [PMID: 26693162]
  5. Br J Nutr. 2022 Feb 14;127(3):344-352 [PMID: 33787474]
  6. World J Gastroenterol. 2014 Nov 7;20(41):15079-86 [PMID: 25386056]
  7. Environ Pollut. 2018 Dec;243(Pt A):492-500 [PMID: 30216881]
  8. Can J Biochem Physiol. 1959 Aug;37(8):911-7 [PMID: 13671378]
  9. J Nutr. 1993 Nov;123(11):1939-51 [PMID: 8229312]
  10. Chemosphere. 2024 Jan;346:140532 [PMID: 37918541]
  11. Cell Metab. 2021 Aug 3;33(8):1685-1700.e9 [PMID: 34237252]
  12. Mol Cell Biochem. 1998 May;182(1-2):169-75 [PMID: 9609126]
  13. Chemosphere. 2019 Feb;217:140-149 [PMID: 30415113]
  14. J Nutr Biochem. 2012 Jun;23(6):557-66 [PMID: 21764281]
  15. Sci Rep. 2024 May 31;14(1):12530 [PMID: 38822155]
  16. Adv Drug Deliv Rev. 2018 Apr;129:54-63 [PMID: 29391222]
  17. Curr Pharm Biotechnol. 2014;15(11):1058-68 [PMID: 25420728]
  18. Gut. 2019 Nov;68(11):2065-2079 [PMID: 31300518]
  19. Biochem Biophys Res Commun. 2017 Nov 4;493(1):227-232 [PMID: 28899784]
  20. Nutr Res. 2017 Aug;44:38-50 [PMID: 28821316]
  21. PLoS One. 2015 Jun 04;10(6):e0129251 [PMID: 26042593]
  22. Environ Health Perspect. 2010 Jun;118(6):796-802 [PMID: 20146964]
  23. Diabetologia. 2007 Sep;50(9):1841-1851 [PMID: 17624515]
  24. Toxicology. 2017 Sep 1;390:124-134 [PMID: 28890136]
  25. Hepatology. 2008 Dec;48(6):1810-20 [PMID: 19030170]
  26. Int J Environ Res Public Health. 2012 May;9(5):1939-53 [PMID: 22754483]
  27. Environ Res. 2007 Mar;103(3):413-8 [PMID: 17187776]
  28. Rev Environ Health. 2023 Jul 12;: [PMID: 37434382]
  29. J Clin Invest. 1999 Jun;103(11):1489-98 [PMID: 10359558]
  30. Methods Mol Biol. 2017;1627:395-407 [PMID: 28836216]
  31. J Hepatol. 2018 Dec;69(6):1335-1348 [PMID: 30142428]
  32. Int J Mol Sci. 2018 Dec 10;19(12): [PMID: 30544653]
  33. Toxicol Appl Pharmacol. 2019 Mar 15;367:71-81 [PMID: 30768972]
  34. Natl Toxicol Program Tech Rep Ser. 2006 Jan;(520):4-246 [PMID: 16628245]
  35. Biochim Biophys Acta. 2009 Jun;1792(6):497-505 [PMID: 19268705]
  36. Diabetes Care. 2007 Mar;30(3):622-8 [PMID: 17327331]
  37. Toxicol Sci. 2017 Dec 1;160(2):256-267 [PMID: 28973532]
  38. Environ Health Perspect. 2010 Dec;118(12):1735-42 [PMID: 21126940]
  39. Environ Health Perspect. 1989 May;81:225-39 [PMID: 2503374]
  40. J Biol Chem. 2000 Sep 15;275(37):28918-28 [PMID: 10844002]
  41. Sci Rep. 2015 Sep 02;5:13729 [PMID: 26330104]
  42. Methods. 2001 Dec;25(4):402-8 [PMID: 11846609]
  43. Environ Health Perspect. 2013 Jan;121(1):105-10 [PMID: 23099484]
  44. Sci Rep. 2017 Jan 12;7:40539 [PMID: 28079158]
  45. Obes Rev. 2011 Aug;12(8):622-36 [PMID: 21457182]
  46. J Biol Chem. 2010 Apr 23;285(17):12604-11 [PMID: 20185821]
  47. Environ Int. 2010 Nov;36(8):918-23 [PMID: 19969354]

MeSH Term

Animals
MicroRNAs
Polychlorinated Biphenyls
Male
Mice, Inbred C57BL
Mice
Liver Cirrhosis
Up-Regulation
Liver
Non-alcoholic Fatty Liver Disease
Environmental Pollutants
Lipid Metabolism

Chemicals

MicroRNAs
Polychlorinated Biphenyls
MIRN34a microRNA, mouse
Mirn155 microRNA, mouse
3,4,5,3',4'-pentachlorobiphenyl
Environmental Pollutants
Journal Article
Article has an altmetric score of 8

Word Cloud

Created with Highcharts 10.0.0PCB126liverfibrosisexposureexpressionmiR-155miR-34apolychlorinatedassociatedsteatohepatitishepaticmiRNAsThereforestudynon-alcoholicC57BL/6miceshowedlipidsteatosisNASHEnvironmentalpollutantsincludingbiphenylsPCBsactendocrinedisruptorsimpairvariousphysiologicalprocessescirrhosisinjuriesdisorderscanregulatedmicroRNAsaimedinvestigaterolefattydiseaseAdultmaleexposed5μmol/kgbodyweight10weeksgroupaccumulationpresencemacro-microvesicularincreasedinflammatoryprofibroticgeneconsistentalsoupregulatedinduceproinflammatorycytokinesinflammationreduceperoxisomeproliferator-activatedreceptorαturnimpairsoxidationpresentinducedviapotentialmechanismsinvolvingmaycontributedevelopmentnewdiagnosticmarkerstherapeuticstrategiesLong-termbiphenylinducesupregulates

Similar Articles

Cited By

No available data.