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Current opinion in cell biology
Oct, 2024;90 102418.

Cell dynamics revealed by microscopy advances.

Max A Hockenberry, Timothy A Daugird, Wesley R Legant
Author Information
  1. Max A Hockenberry: Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, Chapel Hill, NC, USA.
  2. Timothy A Daugird: Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  3. Wesley R Legant: Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Joint Department of Biomedical Engineering, North Carolina State University, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: legantw@email.unc.edu.
PMID: 39159598 DOI: 10.1016/j.ceb.2024.102418

Abstract

Cell biology emerges from spatiotemporally coordinated molecular processes. Recent advances in live-cell microscopy, fueled by a surge in optical, molecular, and computational technologies, have enabled dynamic observations from single molecules to whole organisms. Despite technological leaps, there is still an untapped opportunity to fully leverage their capabilities toward biological insight. We highlight how single-molecule imaging has transformed our understanding of biological processes, with a focus on chromatin organization and transcription in the nucleus. We describe how this was enabled by the close integration of new imaging techniques with analysis tools and discuss the challenges to make a comparable impact at larger scales from organelles to organisms. By highlighting recent successful examples, we describe an outlook of ever-increasing data and the need for seamless integration between dataset visualization and quantification to realize the full potential warranted by advances in new imaging technologies.

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Grants

  1. DP2 GM136653/NIGMS NIH HHS

MeSH Term

Humans
Animals
Microscopy
Chromatin
Cell Nucleus
Single Molecule Imaging

Chemicals

Chromatin
Journal Article Review

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