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Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Dec, 2024;55 (4): 3097-3115.

Production, purification and characterization of phytase from Pichia kudriavevii FSMP-Y17and its application in layers feed.

Ritu Sharma, Arpana Mittal, Varun Gupta, Neeraj K Aggarwal
Author Information
  1. Ritu Sharma: Department of Microbiology, Kurukshetra University, Kurukshetra, 136119, Haryana, India.
  2. Arpana Mittal: Department of Microbiology, Kurukshetra University, Kurukshetra, 136119, Haryana, India.
  3. Varun Gupta: Gobind Ballabh Pant University of Agriculture and Technology, Pant Nagar, Uttarakhand, India.
  4. Neeraj K Aggarwal: Department of Microbiology, Kurukshetra University, Kurukshetra, 136119, Haryana, India. nkumar@kuk.ac.in. ORCID
PMID: 39162933 DOI: 10.1007/s42770-024-01492-x

Abstract

INTRODUCTION: Phytase, recognized for its ability to enhance the nutritional value of phytate-rich foods, has has gained significant prominence. The production of this enzyme has been significantly boosted while preserving economic efficiency by utilizing natural substrates and optimizing essential factors. This study focuses on optimizing phytase production through solid-state fermentation and evaluating its effectiveness in enhancing nutrient utilization in chicken diets.
OBJECTIVE: The objective is to optimize phytase production via solid-state fermentation, characterize purified phytase properties, and assess its impact on nutrient utilization in chicken diets. Through these objectives, we aim to deepen understanding of phytase's role in poultry nutrition and contribute to more efficient feed formulations for improved agricultural outcomes.
METHODOLOGY: We utilized solid-state fermentation with Pichia kudriavzevii FSMP-Y17 yeast on orange peel substrate, optimizing variables like temperature, pH, incubation time, and supplementing with glucose and ammonium sulfate. Following fermentation, we purified the phytase enzyme using standard techniques, characterizing its properties, including molecular weight, optimal temperature and pH, substrate affinity, and kinetic parameters.
RESULTS: The optimized conditions yielded a remarkable phytase yield of 7.0 U/gds. Following purification, the enzyme exhibited a molecular weight of 64 kDa and displayed optimal activity at 55 ��C and pH 5.5, with kinetic parameters (Km���=���3.39��������10 M and a V of 7.092 mM/min) indicating efficient substrate affinity.
CONCLUSION: The addition of purified phytase to chicken diets resulted in significant improvements in nutrient utilization and overall performance, including increased feed intake, improved feed conversion ratio, enhanced bird growth, better phosphorus retention, and improved egg production and quality. By addressing challenges associated with phytate-rich diets, such as reduced nutrient availability and environmental pollution, phytase utilization promotes animal welfare and sustainability in poultry production.

Keywords

Layers Optimization Orange peel Phytase Poultry Solid State fermentation

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MeSH Term

6-Phytase
Pichia
Animals
Animal Feed
Chickens
Fermentation
Phytic Acid
Kinetics
Hydrogen-Ion Concentration
Temperature
Molecular Weight

Chemicals

6-Phytase
Phytic Acid
Journal Article

Word Cloud

Created with Highcharts 10.0.0phytaseproductionfermentationnutrientutilizationdietsfeedenzymeoptimizingsolid-statechickenpurifiedimprovedsubstratepHPhytasephytate-richsignificantpropertiespoultryefficientPichiapeeltemperatureFollowingincludingmolecularweightoptimalaffinitykineticparameters7purification5INTRODUCTION:recognizedabilityenhancenutritionalvaluefoodsgainedprominencesignificantlyboostedpreservingeconomicefficiencyutilizingnaturalsubstratesessentialfactorsstudyfocusesevaluatingeffectivenessenhancingOBJECTIVE:objectiveoptimizeviacharacterizeassessimpactobjectivesaimdeepenunderstandingphytase'srolenutritioncontributeformulationsagriculturaloutcomesMETHODOLOGY:utilizedkudriavzeviiFSMP-Y17yeastorangevariableslikeincubationtimesupplementingglucoseammoniumsulfateusingstandardtechniquescharacterizingRESULTS:optimizedconditionsyieldedremarkableyield0U/gdsexhibited64 kDadisplayedactivity55 ��CKm���=���339��������10 MV092 mM/minindicatingCONCLUSION:additionresultedimprovementsoverallperformanceincreasedintakeconversionratioenhancedbirdgrowthbetterphosphorusretentioneggqualityaddressingchallengesassociatedreducedavailabilityenvironmentalpollutionpromotesanimalwelfaresustainabilityProductioncharacterizationkudriaveviiFSMP-Y17andapplicationlayersLayersOptimizationOrangePoultrySolidState

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