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Sep 04, 2024;13 (17):

PhoP/PhoQ Two-Component System Contributes to Intestinal Inflammation Induced by in Neonatal Mice.

Yan Ma, Yingying Zhang, Yuting Wang, Zhu Qiao, Yingying Liu, Xiaodong Xia
Author Information
  1. Yan Ma: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian 463000, China.
  2. Yingying Zhang: The College of Life Sciences, Northwest University, Xi'an 710068, China.
  3. Yuting Wang: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian 463000, China.
  4. Zhu Qiao: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian 463000, China. ORCID
  5. Yingying Liu: School of Biological and Food Processing Engineering, Huanghuai University, Zhumadian 463000, China.
  6. Xiaodong Xia: State Key Laboratory of Marine Food Processing and Safety Control, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. ORCID
PMID: 39272573 DOI: 10.3390/foods13172808

Abstract

() is a foodborne pathogen capable of causing severe infections in newborns. The PhoP/PhoQ two-component system exerts a significant influence on bacterial virulence. This study aimed to investigate the impact of the PhoP/PhoQ system on intestinal inflammation in neonatal mice induced by . Neonatal mice were infected orally by BAA-894 (WT), a -gene-deletion strain (��), and a complementation strain (��), and the intestinal inflammation in the mice was monitored. Deletion of the gene reduced the viable count of in the ileum and alleviated intestinal tissue damage. Moreover, caspase-3 activity in the ileum of the WT- and ��-infected mice was significantly elevated compared to that of the �� and control groups. ELISA results showed elevated levels of TNF-�� and IL-6 in the ileum of the mice infected with WT and ��. In addition, deletion of the gene in resulted in a down-regulation of inflammatory genes (IL-1��, TNF-��, IL-6, NF-��B p65, TLR4) within the ileum and decreased inflammation by modulating the TLR4/NF-��B pathway. It is suggested that targeting the PhoP/PhoQ two-component system could be a potential strategy for mitigating -induced neonatal infections.

Keywords

Cronobacter sakazakii PhoP/PhoQ system intestinal inflammation neonatal mice

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Grants

  1. 2022YFD2100104/the National Key Research and Development Program of China
  2. 242102110136/he Science and Technology Development Plan Project of Henan Province, China
  3. 242102110117/the Science and Technology Development Plan Project of Henan Province, China
  4. 24B550006/Key scientific research project of Henan Higher Education Institutions
  5. XKPY-2023001/National Scientific Research Project Cultivation Fund project of Huanghuai University
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