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2024;5 0064.

Synergistic Assembly of 1DZnO and Anti-CYFRA 21-1: A Physicochemical Approach to Optical Biosensing.

Rafael A Salinas, Shirlley E Martínez Tolibia, Patricia G Zayas-Bazán, Sandra E Rodil, Mathew T Mathew, Andrés Navarrete, Guillermo Santana, Ateet Dutt
Author Information
  1. Rafael A Salinas: Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  2. Shirlley E Martínez Tolibia: Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  3. Patricia G Zayas-Bazán: Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  4. Sandra E Rodil: Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  5. Mathew T Mathew: Department of Biomedical Science, UIC College of Medicine, Rockford, IL 61107, USA.
  6. Andrés Navarrete: Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México, Ciudad Universitaria C.P. 04510, Coyoacán, Mexico City, México.
  7. Guillermo Santana: Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  8. Ateet Dutt: Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México. ORCID
PMID: 39297176 DOI: 10.34133/bmef.0064

Abstract

We conducted a comprehensive physicochemical analysis of one-dimensional ZnO nanowires (1DZnO), incorporating anti-CYFRA 21-1 immobilization to promote fast optical biomarker detection up to 10 ng ml. This study highlights the effectiveness of proof-of-concept 1DZnO nanoplatforms for rapid cancer biomarker detection by examining the nanoscale integration of 1DZnO with these bioreceptors to deliver reliable photoluminescent output signals. The urgent need for swift and accurate prognoses in healthcare settings drives the rise of sensitive biosensing nanoplatforms for cancer detection, which has benefited from biomarker identification. CYFRA 21-1 is a reliable target for the early prediction of cancer formation that can be perceptible in blood, saliva, and serum. However, 1DZnO nanostructures have been barely applied for CYFRA 21-1 detection. We assessed the nanoscale interaction between 1DZnO and anti-CYFRA 21-1 antibodies to develop rapid CYFRA 21-1 detection in two distinct matrices: PhosphateBuffered Saline (PBS) buffer and artificial saliva. The chemical modifications were tracked utilizing Fourier transform infrared spectroscopy, while transmission electron microscopy and energy dispersive spectroscopy confirmed antigen-antibody interplay over nanostructures. Our results show high antibody immobilization efficiencies, affirming the effectiveness of 1DZnO nanoplatforms for rapid CYFRA 21-1 testing within a 5-min detection window in both PBS and artificial saliva. Photoluminescence measurements also revealed distinct optical responses across biomarker concentrations ranging from 10 to 1,000 ng ml. Discernible PL signal responses obtained after 5 min affirm the potential of 1DZnO nanoplatforms for further advancement in optical biomarker detection for application in early cancer prognosis.

References

  1. Mikrochim Acta. 2023 May 23;190(6):235 [PMID: 37219635]
  2. J Mater Chem B. 2020 May 21;8(19):4228-4236 [PMID: 32330210]
  3. J Oral Maxillofac Pathol. 2023 Jan-Mar;27(1):98-102 [PMID: 37234320]
  4. Biomed Mater Devices. 2023 Jun 6;:1-18 [PMID: 37363139]
  5. Chem Commun (Camb). 2019 Aug 20;55(68):10060-10063 [PMID: 31328750]
  6. Int J Oral Maxillofac Surg. 2020 Aug;49(8):973-983 [PMID: 32035907]
  7. J Pharm Sci. 2015 Feb;104(2):750-9 [PMID: 25231267]
  8. Natl J Maxillofac Surg. 2016 Jan-Jun;7(1):17-20 [PMID: 28163473]
  9. J Photochem Photobiol B. 2014 Jan 5;130:153-60 [PMID: 24333763]
  10. Ann Glob Health. 2014 Sep-Oct;80(5):412-7 [PMID: 25512156]
  11. Int J Med Sci. 2012;9(3):193-9 [PMID: 22408567]
  12. Adv Biochem Eng Biotechnol. 2009;116:99-123 [PMID: 19475374]
  13. Nanomaterials (Basel). 2020 Apr 29;10(5): [PMID: 32365564]
  14. Nat Med. 2022 Apr;28(4):649-657 [PMID: 35440716]
  15. J Clin Oncol. 2008 Aug 20;26(24):4012-21 [PMID: 18711192]
  16. JAMA Oncol. 2023 Apr 1;9(4):465-472 [PMID: 36821107]
  17. CA Cancer J Clin. 2021 May;71(3):209-249 [PMID: 33538338]
  18. Nanoscale Res Lett. 2020 Jan 15;15(1):12 [PMID: 31940100]
  19. Science. 2022 Mar 18;375(6586):eaay9040 [PMID: 35298272]
  20. Curr Opin Biomed Eng. 2023 Dec;28: [PMID: 38406798]
  21. J Cancer Res Ther. 2018 Jun;14(Supplement):S400-S404 [PMID: 29970696]
  22. Genes (Basel). 2021 Feb 27;12(3): [PMID: 33673461]
  23. Clin Cancer Res. 2006 Jul 1;12(13):3979-84 [PMID: 16818695]
  24. Mater Sci Eng C Mater Biol Appl. 2015 Aug;53:104-10 [PMID: 26042696]
  25. Analyst. 2016 Feb 7;141(3):740-55 [PMID: 26583164]
  26. J Cancer Res Ther. 2020 Apr-Jun;16(3):452-457 [PMID: 32719250]
  27. Langmuir. 2006 Jun 20;22(13):5804-9 [PMID: 16768511]
  28. J Clin Exp Hepatol. 2011 Jun;1(1):6-12 [PMID: 22228935]
  29. Oral Dis. 2015 Jan;21(1):90-6 [PMID: 24304502]
  30. Adv Lab Med. 2020 Oct 8;1(4):20200005 [PMID: 37360615]
Journal Article
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