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Journal of assisted reproduction and genetics
Nov, 2024;41 (11): 3105-3117.

CATSPER2 and SPEF2 are potential molecular markers for boar sperm quality: a population association study.

Zulfiqar Ahmed, Min Liu, Shuntao Huang, Dequan Xu
Author Information
  1. Zulfiqar Ahmed: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubie, People's Republic of China.
  2. Min Liu: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubie, People's Republic of China.
  3. Shuntao Huang: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubie, People's Republic of China.
  4. Dequan Xu: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubie, People's Republic of China. dequanxu@126.com. ORCID
PMID: 39312031 DOI: 10.1007/s10815-024-03252-0

Abstract

PURPOSE: This study investigates the role of cation channel sperm associated 2 (CATSPER2) and sperm flagella 2 (SPEF2) genes in boar spermatogenesis, focusing on their association with sperm quality traits in boars.
METHODS: Utilizing targeted next-generation sequencing, we identified and genotyped two polymorphisms in CATSPER2 (rs341636020G���>���A, rs326912346G���>���T) and three variants in SPEF2 (rs320839956A���>���G, rs334209514C���>���A, rs325319860C���>���T) across three boar breeds (Duroc, n���=���181; Landrace, n���=���87; Large White, n���=���52).
RESULTS: Our results confirmed the presence of the specified single nucleotide polymorphisms (SNPs), adhering to association study criteria. In CATSPER2, significant associations were detected between rs341636020G���>���A and sperm curvilinear velocity (VCL) in Duroc and Landrace boars, and between rs326912346G���>���T and straight velocity (VSL) in Duroc and Large White boars. For SPEF2, rs320839956A���>���G was significantly linked to sperm viability in Duroc and Landrace and to sperm concentration (SCON) in Large White boars. Additionally, rs334209514C���>���A and rs325319860C���>���T showed significant associations with SCON and VCL respectively, in Doruc and Landrace boars.
CONCLUSIONS: Overall, our findings suggest that CATSPER2 and SPEF2 SNPs significantly impact boar sperm quality traits. These genetic markers have the potential to enhance boar fertility through selective breeding programs, contributing to the optimization of reproductive performance in pigs.

Keywords

Association Molecular markers Polymorphism Spermatogenesis Traits

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Grants

  1. 2021YFD1301201/National Key Research and Development Program of China
  2. 2019-620-000-001-30/Hubei Provincial Key Laboratory of Metallurgical Industry Process System Science
  3. S2021AB3424/Science and Technology Project of Xinjiang Production and Construction Corps

MeSH Term

Male
Animals
Polymorphism, Single Nucleotide
Spermatozoa
Swine
Sperm Motility
Semen Analysis
Spermatogenesis
Genotype
Genetic Association Studies
Sperm Count
Calcium Channels
Breeding

Chemicals

Calcium Channels
Journal Article

Word Cloud

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