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Heliyon
Sep 30, 2024;10 (18): e37931.

in C3H/HeN mice: A model for studying pathogen-host interactions.

Apolline Maitre, Lourdes Mateos-Hernandez, Tal Azagi, Angélique Foucault-Simonin, Sabine Rakotobe, Zbigniew Zając, Pavle Banović, Stefania Porcelli, Aurélie Heckmann, Clémence Galon, Hein Sprong, Sara Moutailler, Alejandro Cabezas-Cruz, Andrea C Fogaça
Author Information
  1. Apolline Maitre: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  2. Lourdes Mateos-Hernandez: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  3. Tal Azagi: National Institute for Public Health and the Environment, Netherlands.
  4. Angélique Foucault-Simonin: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  5. Sabine Rakotobe: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  6. Zbigniew Zając: Department of Biology and Parasitology, Medical University of Lublin, Radziwiłłowska 11 st, 20-080, Lublin, Poland.
  7. Pavle Banović: Clinic for Lyme Borreliosis and Other Tick-Borne Diseases, Pasteur Institute Novi Sad, 21000 Novi Sad, Serbia.
  8. Stefania Porcelli: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  9. Aurélie Heckmann: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  10. Clémence Galon: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  11. Hein Sprong: National Institute for Public Health and the Environment, Netherlands.
  12. Sara Moutailler: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  13. Alejandro Cabezas-Cruz: Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.
  14. Andrea C Fogaça: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
PMID: 39323843 DOI: 10.1016/j.heliyon.2024.e37931

Abstract

An infection with the tick-borne has been associated with a broad spectrum of clinical manifestations in humans, but patients are only seldomly reported. Understanding its disease etiology necessitates well-stablished infection models, improving to recognize and diagnose patients with infection and facilitating the development of effective control strategies. In this study, we used C3H/HeN mice as a model to establish infection, achieving a high infection prevalence (89-100 %). While the liver and the spleen DNA consistently tested positive for infection in all challenged mice, additional infected organs included the kidneys, heart, and the lungs. Notably, a low prevalence of infection was observed in nymphs fed on -challenged mice. In addition, larvae were refractory to infection, suggesting that ticks exhibit low susceptibility to the pathogen. To the best of our knowledge, this is the first study of an animal model for infection. It serves as a valuable tool for advancing research on the interactions among the bacterium and its vertebrate host.

Keywords

Animal models of infection Pathogen Rickettsia helvetica Tick Virulence

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Journal Article

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