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Aug 26, 2024;14 (9):

3D Models Currently Proposed to Investigate Human Skin Aging and Explore Preventive and Reparative Approaches: A Descriptive Review.

Francesca Lombardi, Francesca Rosaria Augello, Alessia Ciafarone, Valeria Ciummo, Serena Altamura, Benedetta Cinque, Paola Palumbo
Author Information
  1. Francesca Lombardi: Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. ORCID
  2. Francesca Rosaria Augello: Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. ORCID
  3. Alessia Ciafarone: Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. ORCID
  4. Valeria Ciummo: Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" of Chieti-Pescara, 66100 Chieti, Italy. ORCID
  5. Serena Altamura: Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. ORCID
  6. Benedetta Cinque: Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
  7. Paola Palumbo: Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. ORCID
PMID: 39334833 DOI: 10.3390/biom14091066

Abstract

Skin aging is influenced by intrinsic and extrinsic factors that progressively impair skin functionality over time. Investigating the skin aging process requires thorough research using innovative technologies. This review explores the use of in vitro human 3D culture models, serving as valuable alternatives to animal ones, in skin aging research. The aim is to highlight the benefits and necessity of improving the methodology in analyzing the molecular mechanisms underlying human skin aging. Traditional 2D models, including monolayers of keratinocytes, fibroblasts, or melanocytes, even if providing cost-effective and straightforward methods to study critical processes such as extracellular matrix degradation, pigmentation, and the effects of secretome on skin cells, fail to replicate the complex tissue architecture with its intricated interactions. Advanced 3D models (organoid cultures, "skin-on-chip" technologies, reconstructed human skin, and 3D bioprinting) considerably enhance the physiological relevance, enabling a more accurate representation of skin aging and its peculiar features. By reporting the advantages and limitations of 3D models, this review highlights the importance of using advanced in vitro systems to develop practical anti-aging preventive and reparative approaches and improve human translational research in this field. Further exploration of these technologies will provide new opportunities for previously unexplored knowledge on skin aging.

Keywords

3D bioprinting 3D skin models aging human skin pseudo-3D system reconstructed human skin skin microbiota skin organoids skin-on-chip

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Grants

  1. FFO MeSVA 2022/Department of Life, Health, and Environmental Sciences, University of L'Aquila
  2. FFO MeSVA 2023/Department of Life, Health, and Environmental Sciences, University of L'Aquila

MeSH Term

Humans
Skin Aging
Skin
Melanocytes
Keratinocytes
Fibroblasts
Models, Biological
Printing, Three-Dimensional
Bioprinting
Cell Culture Techniques, Three Dimensional
Journal Article Review

Word Cloud

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