Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of and Anaplasma boleense in Paraguay.
Oscar Daniel Salvioni Recalde, Miriam Soledad Rol��n, Myriam Celeste Vel��zquez, Martin M Kowalewski, Jorge Javier Alfonso Ruiz Diaz, Antonieta Rojas de Arias, Milton Oz��rio Moraes, Harrison Magdinier Gomes, Bruna de Azevedo Ba��ta, Matheus Dias Cordeiro, Mar��a Celeste Vega G��mez
Author Information
Oscar Daniel Salvioni Recalde: Center for the Development of Scientific Research (CEDIC), Manduvira 635, Asunci��n 1255, Paraguay. ORCID
Miriam Soledad Rol��n: Center for the Development of Scientific Research (CEDIC), Manduvira 635, Asunci��n 1255, Paraguay. ORCID
Martin M Kowalewski: Estaci��n Biol��gica Corrientes CECOAL (Centro de Ecolog��a Aplicada del Litoral) CONICET-UNNE, Corrientes 3400, Argentina. ORCID
Jorge Javier Alfonso Ruiz Diaz: Center for the Development of Scientific Research (CEDIC), Manduvira 635, Asunci��n 1255, Paraguay. ORCID
Antonieta Rojas de Arias: Center for the Development of Scientific Research (CEDIC), Manduvira 635, Asunci��n 1255, Paraguay.
Milton Oz��rio Moraes: Programa de P��s-Gradua����o em Biologia Celular e Molecular, Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
Harrison Magdinier Gomes: Laborat��rio de Biologia Molecular Aplicada �� Micobact��rias, Instituto Oswaldo Cruz (IOC), Funda����o Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Manguinhos 21045-900, Brazil.
Bruna de Azevedo Ba��ta: Laboratory of Parasitic Diseases of the Federal Rural University of Rio de Janeiro (UFRRJ), Km 07, Serop��dica, Rio de Janeiro 21040-900, Brazil.
Matheus Dias Cordeiro: Laboratory of Parasitic Diseases of the Federal Rural University of Rio de Janeiro (UFRRJ), Km 07, Serop��dica, Rio de Janeiro 21040-900, Brazil.
Mar��a Celeste Vega G��mez: Center for the Development of Scientific Research (CEDIC), Manduvira 635, Asunci��n 1255, Paraguay. ORCID
Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR. All amplified products were subsequently sequenced. The bacteria detected in the blood hosts included , , and , Anaplasma boleense, and spp., which had not been previously reported in the country. Free-living and parasitic ticks on dogs () and wild armadillos () were collected and identified as and spp. The species , , , and . A. boleense were detected in domestic dog ticks, and and were found for the first time in armadillos and free-living ticks. Blood feeding sources detected in free-living ticks were rodents, humans, armadillos and dogs. Results show a high diversity of tick-borne pathogens circulating among domestic and wild animals in the northeastern region of Paraguay.
