Uddipan Dasgupta: Amity Institute of Nanotechnology, Amity University Kolkata, Major Arterial Road, AA II, Newtown, Kolkata 700135, West Bengal, India.
Malabika Ghosh: Amity Institute of Nanotechnology, Amity University Kolkata, Major Arterial Road, AA II, Newtown, Kolkata 700135, West Bengal, India.
Rupali Gangopadhyay: Department of Chemistry, Sister Nivedita University, Action Area I, DG Block, 1/2, New Town, Kolkata 700156, West Bengal, India.
Nguyen Thi Ngoc Anh: Institute of Analytical and Environmental Sciences, National Tsing Hua University, 101, Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan, ROC.
Ruey-An Doong: Institute of Analytical and Environmental Sciences, National Tsing Hua University, 101, Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan, ROC. ORCID
Provash Chandra Sadhukhan: Division of Virus Laboratory, ICMR-National Institute of Cholera and Enteric Diseases (NICED), Kolkata 700010, India. ORCID
Ankan Dutta Chowdhury: Amity Institute of Nanotechnology, Amity University Kolkata, Major Arterial Road, AA II, Newtown, Kolkata 700135, West Bengal, India. ORCID
A combination of magnetic and noble metal nanoparticles (NPs) has recently emerged as a potential substance for rapid and sensitive immunosorbent assays. However, to make the assay an alternative method for Enzyme-linked immunosorbent assay, the individual role of each nanoparticle must be explored properly. In this work, an immunoassay has been proposed using two antibody-conjugated iron oxide nanoparticles (FeONPs) and gold-silver bimetallic nanoparticles (AuAgNPs) to enhance the sensitivity of virus detection by colorimetric TMB/HO signal amplification. A synergistic effect is monitored between FeONPs and AuAgNPs, which is explored for colorimetric virus detection. The sensor exploits the synergistic effect between the nanoparticles to successfully detect a wide range of dengue virus-like particle (DENV-LP) concentrations ranging from 10 to 100 pg/mL with a detection limit of up to 2.6 fg/mL. In the presence of a target DENV-LP, a sandwich-like structure is formed, which restricts the electron transfer and the associated synergistic effect between the nanoparticles, restricting the TMB oxidation process. Therefore, the synergistic effect is the key to the present work, which accounts for the enhanced rate of the enzymatic reaction on TMB and makes the current method of virus detection more sensitive and reliable compared to the others.
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