Synergistic efficacy of ZnO quantum dots, Ag NPs, and nitazoxanide composite against multidrug-resistant human pathogens as new trend of revolutionizing antimicrobial treatment.
Islam Gomaa, Ghadah Aleid, Shahira H El-Moslamy, Anoud AlShammari, Sumayyah Al-Marshedy, Freah Alshammary, Jouza Gharkan, Reda Abdel-Hameed, Elbadawy A Kamoun
Author Information
Islam Gomaa: Nanotechnology Research Centre (NTRC), The British University in Egypt (BUE), Suez Desert Road, El-Sherouk City, Cairo, 11837, Egypt.
Ghadah Aleid: Basic Science Departments, Preparatory Year, University of Ha'il, 1560, Hail, Kingdom of Saudi Arabia.
Shahira H El-Moslamy: Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab City, Alexandria, 21934, Egypt.
Anoud AlShammari: Department of Physics and Chemistry, Northern Border University, Rafha, Kingdom of Saudi Arabia.
Sumayyah Al-Marshedy: Biochemistry Department, College of Medicine, University of Ha'il, Hail, Kingdom of Saudi Arabia.
Freah Alshammary: Department of Preventive Dental Sciences, College of Dentistry, University of Ha'il, Hail, Kingdom of Saudi Arabia.
Jouza Gharkan: Emergency Medical Services and Critical Care, Inaya Medical College, Riyadh, Kingdom of Saudi Arabia.
Reda Abdel-Hameed: Basic Science Departments, Preparatory Year, University of Ha'il, 1560, Hail, Kingdom of Saudi Arabia. mredars@yahoo.com.
Elbadawy A Kamoun: Department of Chemistry, College of Science, King Faisal University, 31982, Al-Ahsa, Kingdom of Saudi Arabia. ekamoun@kfu.edu.sa.
Antibiotic resistance is currently becoming a more serious threat to global health, especially in severe nosocomial infections treatment by multidrug-resistant bacteria. This research provides a new way of synergizing green-synthesis for zinc oxide quantum dots (ZnO-QDs with hexagonal crystals) that are 7 nm in diameter and zero-valent Ag cubic crystals that are 67 nm in size embedded with nitazoxanide substrate (NAZ). Instrumental characterization like SEM, TEM, EDAX, and FT-IR and comprehensive antimicrobial studies were conducted to study the incorporation behavior of composites based on Ag NPs/ZnO QDs/NAZ. This combination has not been hitherto addressed anywhere else in the published literature, as well as commercial viability. In this context, we have precisely tuned nanoparticle to nitazoxanide ratio for designing the formulation demonstrating potent activity against MDR infections. By employing nitazoxanide as a scaffold and careful decoration thereof antimicrobial potency has been unlocked overriding conventional therapies. In addition, Ag NPs/ZnO-QDs/nitazoxanide (G6) formula exhibited a therapeutic efficacy span of 96.15��������1.68% to 99.57��������0.20% against MDR human infections post 48 h incubation; a breakthrough in therapeutic efficacy levels has been achieved by our method. Accordingly, ZnO QDs/Ag NPs/NAZ composite offered potential multidrug resistant human pathogens as a new trend of revolutionizing antimicrobial treatment.
