Inhalation of 2,2',5,5'-tetrachlorobiphenyl (PCB52) causes changes to the gut microbiome throughout the gastrointestinal tract.
Laura E Dean, Hui Wang, Amanda J Bullert, Hui Wang, Andrea Adamcakova-Dodd, Ashutosh K Mangalam, Peter S Thorne, James A Ankrum, Aloysius J Klingelhutz, Hans-Joachim Lehmler
Author Information
Laura E Dean: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States. Electronic address: laura-gosse@uiowa.edu.
Hui Wang: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States. Electronic address: hui-wang-2@uiowa.edu.
Amanda J Bullert: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States; Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, IA, United States. Electronic address: amanda-bullert@uiowa.edu.
Hui Wang: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States. Electronic address: hui-wang-1@uiowa.edu.
Andrea Adamcakova-Dodd: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States. Electronic address: andrea-a-dodd@uiowa.edu.
Ashutosh K Mangalam: Department of Pathology, University of Iowa, Iowa City, IA, United States. Electronic address: ashutosh-mangalam@uiowa.edu.
Peter S Thorne: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States; Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, IA, United States. Electronic address: peter-thorne@uiowa.edu.
James A Ankrum: Department of Biomedical Engineering, University of Iowa, Iowa City, IA, United States. Electronic address: james-ankrum@uiowa.edu.
Aloysius J Klingelhutz: Department of Microbiology and Immunology, University of Iowa, Iowa City, IA, United States. Electronic address: al-klingelhutz@uiowa.edu.
Hans-Joachim Lehmler: Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA, United States; Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, IA, United States. Electronic address: hans-joachim-lehmler@uiowa.edu.
Polychlorinated biphenyls (PCBs), such as PCB52, are hazardous environmental contaminants present in indoor and outdoor environments. Oral PCB exposure affects the colon microbiome; however, it is unknown if inhalation of PCBs alters the intestinal microbiome. We hypothesize that sub-acute inhalation of PCB52 affects microbial communities depending on the location in the (GI) gastrointestinal tract and the local profiles of PCB52 and its metabolites present in the GI tract following mucociliary clearance and biliary or intestinal excretion. Sprague-Dawley rats were exposed via nose-only inhalation 4 h per day, 7 days per week, for 4 weeks to either filtered air or PCB52. After 28 days, differences in the microbiome and levels of PCB52 and its metabolites were characterized throughout the GI tract. PCB52 inhalation altered taxa abundances and predicted functions altered throughout the gut, with most alterations occurring in the large intestine. PCB52 and metabolite levels varied across the GI tract, resulting in differing PCB × microbiome networks. Thus, the presence of different levels of PCB52 and its metabolites in different parts of the GI tract has varying effects on the composition and predicted function of microbial communities. Future studies need to investigate whether these changes lead to adverse outcomes.
