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Nov, 2024;14 (11): 1805-1824.

Cylindracin, a Cys-rich protein expressed in the fruiting body of Cyclocybe cylindracea, inhibits growth of filamentous fungi but not yeasts or bacteria.

Yamato Kuratani, Chika Abematsu, Keisuke Ekino, Takuji Oka, Masashi Shin, Makoto Iwata, Hiroto Ohta, Shoji Ando
Author Information
  1. Yamato Kuratani: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan.
  2. Chika Abematsu: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan.
  3. Keisuke Ekino: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan.
  4. Takuji Oka: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan. ORCID
  5. Masashi Shin: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan.
  6. Makoto Iwata: IMB Co., Ltd., Asakura, Japan.
  7. Hiroto Ohta: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan.
  8. Shoji Ando: Faculty of Biotechnology and Life Science, Sojo University, Kumamoto, Japan. ORCID
PMID: 39380157 DOI: 10.1002/2211-5463.13910

Abstract

Mushrooms are the fruiting bodies of fungi and are important reproductive structures that produce and disseminate spores. The Pri3 gene was originally reported to be specifically expressed in the primordia (a precursor to the mature fruiting body) of the edible mushroom Cyclocybe aegerita. Here, we cloned a Pri3-related cDNA from Cyclocybe cylindracea, another species in the same genus, and showed that the gene is specifically expressed at the pileus surface of the immature fruiting body but not in the primordia. Immunohistochemistry showed that the translated protein is secreted into a polysaccharide layer of the pileus surface. The recombinant C-terminal Cys-rich domain of the protein showed antifungal activity against three filamentous fungi and inhibited hyphal growth and conidiogenesis. These results suggest that the PRI3-related protein of C. cylindracea, named cylindracin, plays an important role in the defense against pathogens.

Keywords

Basidiomycetes Cyclocybe cylindracea Pri3 antifungal protein cylindracin host defense

References

  1. Mol Microbiol. 2006 Mar;59(5):1380-94 [PMID: 16468983]
  2. Peptides. 2005 Feb;26(2):191-6 [PMID: 15629530]
  3. Biophys J. 2001 Feb;80(2):952-60 [PMID: 11159462]
  4. J Biochem. 1998 Jul;124(1):141-7 [PMID: 9644256]
  5. Protein Eng. 1997 Jan;10(1):1-6 [PMID: 9051728]
  6. Biochemistry. 2017 Sep 19;56(37):4992-5001 [PMID: 28825809]
  7. FEBS Lett. 1989 Nov 20;258(1):75-8 [PMID: 2556299]
  8. Biochim Biophys Acta. 1981 Sep 15;661(1):136-41 [PMID: 7028121]
  9. FEMS Yeast Res. 2018 Feb 1;18(1): [PMID: 29346565]
  10. Foods. 2016 Nov 29;5(4): [PMID: 28231175]
  11. J Microbiol Biotechnol. 2022 Jan 28;32(1):37-45 [PMID: 34750288]
  12. Science. 2010 May 28;328(5982):1168-72 [PMID: 20508130]
  13. Sci Rep. 2018 Nov 16;8(1):16918 [PMID: 30446686]
  14. Cell Mol Life Sci. 2006 Jun;63(12):1370-85 [PMID: 16715411]
  15. Fungal Genet Biol. 2011 Apr;48(4):359-69 [PMID: 21281728]
  16. Microbiol Spectr. 2017 May;5(3): [PMID: 28513415]
  17. Biochemistry. 1997 Feb 18;36(7):1912-7 [PMID: 9048578]
  18. Cell Mol Life Sci. 2017 Feb;74(4):663-682 [PMID: 27557668]
  19. Nutrients. 2020 Feb 13;12(2): [PMID: 32069862]
  20. Arch Biochem Biophys. 1959 May;82(1):70-7 [PMID: 13650640]
  21. Stud Mycol. 2023 Jul;104:1-85 [PMID: 37351542]
  22. J Agric Food Chem. 2002 Oct 23;50(22):6419-22 [PMID: 12381127]
  23. Microbiology (Reading). 2008 Sep;154(Pt 9):2730-2739 [PMID: 18757806]
  24. J Biol Chem. 2014 Dec 12;289(50):34953-64 [PMID: 25342741]
  25. Bioessays. 2006 Aug;28(8):799-808 [PMID: 16927300]
  26. Genomics Proteomics Bioinformatics. 2020 Jun;18(3):341-351 [PMID: 32561469]
  27. Biopolymers. 2002;66(4):236-48 [PMID: 12491537]
  28. PLoS One. 2013;8(2):e56686 [PMID: 23418592]
  29. Mol Cell Biol. 1987 Dec;7(12):4390-9 [PMID: 3325823]
  30. J Bacteriol. 1999 Jun;181(12):3721-9 [PMID: 10368147]
  31. J Antibiot (Tokyo). 2020 Oct;73(10):657-665 [PMID: 32684620]
  32. Fungal Genet Biol. 1997 Feb;21(1):141-52 [PMID: 9126623]
  33. J Biol Chem. 1996 Feb 23;271(8):4168-76 [PMID: 8626758]
  34. Sci Rep. 2018 Jun 12;8(1):8983 [PMID: 29895888]
  35. Biochim Biophys Acta. 2012 Jul;1818(7):1648-55 [PMID: 22406553]
  36. FEMS Microbiol Lett. 2006 Sep;262(1):1-8 [PMID: 16907732]
  37. Nutrition. 2000 Jul-Aug;16(7-8):694-6 [PMID: 10906601]
  38. Curr Genet. 1991 Aug;20(3):205-10 [PMID: 1718610]
  39. BMC Res Notes. 2011 Oct 28;4:459 [PMID: 22032337]
  40. Toxins (Basel). 2022 Jan 22;14(2): [PMID: 35202112]
  41. Appl Environ Microbiol. 1995 Apr;61(4):1614-9 [PMID: 7747975]
  42. J Food Prot. 1990 Oct;53(10):883-894 [PMID: 31018285]
  43. Curr Genet. 2002 Apr;41(1):31-42 [PMID: 12073098]
  44. Anal Biochem. 1985 Aug 15;149(1):229-37 [PMID: 4073479]
  45. Appl Microbiol Biotechnol. 2000 Aug;54(2):141-52 [PMID: 10968625]
  46. Proc Natl Acad Sci U S A. 1950 Feb;36(2):102-6 [PMID: 16588952]
  47. Microbiol Mol Biol Rev. 2000 Jun;64(2):316-53 [PMID: 10839819]
  48. J Sci Food Agric. 2013 Jan;93(2):209-18 [PMID: 23172575]
  49. ISME J. 2019 Mar;13(3):588-602 [PMID: 30301946]
  50. World J Microbiol Biotechnol. 2016 Mar;32(3):35 [PMID: 26861586]
  51. Biotechnol Rep (Amst). 2019 Nov 21;25:e00392 [PMID: 31871922]
  52. Planta Med. 2021 Apr;87(5):351-367 [PMID: 33063304]
  53. Biochem Soc Trans. 2022 Feb 28;50(1):423-437 [PMID: 35015081]
  54. J Biol Chem. 2010 Nov 26;285(48):37513-20 [PMID: 20861017]
  55. Semin Cell Dev Biol. 2019 Apr;88:107-118 [PMID: 29432955]

Grants

  1. /Sojo University

MeSH Term

Fruiting Bodies, Fungal
Fungal Proteins
Agaricales
Antifungal Agents
Bacteria
Yeasts
Fungi
Amino Acid Sequence
Cysteine

Chemicals

Fungal Proteins
Antifungal Agents
Cysteine
Journal Article
Article has an altmetric score of 1

Word Cloud

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