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10 23, 2024;14 (1): 24998.

Development of a 17-plex STR typing system for the identification of individuals and parentage testing in cattle.

Songyang Shang, Yutong Wang, Xiujuan Yu, Defu Zhang, Runhong Luo, Ri Jiang, Gang Zhao, Xuehai Du, Jupeng Zhang, David M Irwin, Zhe Wang, Shuyi Zhang
Author Information
  1. Songyang Shang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  2. Yutong Wang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  3. Xiujuan Yu: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  4. Defu Zhang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  5. Runhong Luo: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  6. Ri Jiang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  7. Gang Zhao: Liaoning Agricultural Development Service Center, Shenyang, 110032, Liaoning, China.
  8. Xuehai Du: Liaoning Agricultural Development Service Center, Shenyang, 110032, Liaoning, China.
  9. Jupeng Zhang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  10. David M Irwin: Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.
  11. Zhe Wang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China.
  12. Shuyi Zhang: College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China. szhang@syau.edu.cn.
PMID: 39443655 DOI: 10.1038/s41598-024-76547-y

Abstract

Accurate identification of animals and the verification of their parentage can be used to pedigree populations and support selective breeding. The International Society for Animal Genetics recommended 16 cattle STRs for individual identification and parentage testing in cattle, but no multiplex STR typing system contains these 16 STRs. Here, we develop an efficient 17-plex multiplex typing system for cattle that contains the 16 ISAG recommend STRs and a sex-determining marker. Compared to the Bovine Parenting Typing Kit (containing 11 of the 16 ISAG recommend STRs), our new typing system not only increases the number of molecular markers, but also simplifies the PCR operation and shortens the time for the typing procedure (from 4.5 h to 1 h 37 min). Profile can be generated from a single PCR reaction using as little as 1 ng of DNA. The combined probabilities of paternity exclusion CPE and CPE were 0.999804697 and 0.999999260, respectively. These results indicate that our 17-plex typing system is a fast, sensitive and species-specific method for the identification of individuals and their parentage for cattle. The application of this system will improve the efficiency of the identification of cattle individuals and their paternity, supporting population genetic research and the selective breeding of cattle.

Keywords

Cattle Microsatellite Multiplex PCR Parentage testing

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Grants

  1. JYTYB2024026/Department of Education of Liaoning Province

MeSH Term

Animals
Cattle
Microsatellite Repeats
Pedigree
Female
Male
Multiplex Polymerase Chain Reaction
Journal Article

Word Cloud

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