Anti-Photoaging Effects of Antioxidant Peptide from Seahorse () in In Vivo and In Vitro Models.
Fengqi Yang, Yang Yang, Dandan Xiao, Poongho Kim, Jihee Lee, You-Jin Jeon, Lei Wang
Author Information
Fengqi Yang: Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea. ORCID
Yang Yang: State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
Dandan Xiao: Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea. ORCID
Poongho Kim: South Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Republic of Korea.
Jihee Lee: South Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Republic of Korea. ORCID
You-Jin Jeon: Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea. ORCID
Lei Wang: State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China. ORCID
Overexposure to ultraviolet (UV) radiation can lead to photoaging, which contributes to skin damage. The objective of this study was to evaluate the effects of an antioxidant peptide (SHP2) purified from seahorse () alcalase hydrolysate on UVB-irradiated skin damage in human keratinocyte (HaCaT) and human dermal fibroblast (HDF) cells and a zebrafish model. The data revealed that SHP2 significantly enhanced cell viability by attenuating apoptosis through the reduction of intracellular reactive oxygen species (ROS) levels in UVB-stimulated HaCaT cells. Moreover, SHP2 effectively inhibited ROS, improved collagen synthesis, and suppressed the secretion of matrix metalloproteinases (MMPs) in UVB-irradiated HDF cells. SHP2 restored the protein levels of HO-1, Nrf2, and SOD, while decreasing Keap1 expression in UVB-treated HDF, indicating stimulation of the Keap1/Nrf2/HO-1 signaling pathway. Furthermore, an in vivo study conducted in zebrafish confirmed that SHP2 inhibited photoaging by reducing cell death through the suppression of ROS generation and lipid peroxidation. Particularly, 200 µg/mL of SHP2 exerted a remarkable anti-photoaging effect on both in vitro and in vivo models. These results demonstrate that SHP2 possesses antioxidant properties and regulates skin photoaging activities, suggesting that SHP2 may have the potential for use in the development of cosmetic products.
