The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study.
Yury A Novosad, Anton S Shabunin, Natella I Enukashvily, Olga V Supilnikova, Anastasia I Konkina, Natalia Yu Semenova, Gleb S Yatsemirsky, Evgenii V Zinoviev, Kristina N Rodionova, Kirill L Kryshen, Antonina Yu Borodina, Alexander Yu Makarov, Andrey M Fedyuk, Alexander D Nilov, Elena V Chikulaeva, Lidiya S Konkova, Irina S Chustrak, Veronika V Traxova, Platon A Safonov, Sergey V Vissarionov, Egor M Prikhodko, Yury V Yurkevich
Author Information
Yury A Novosad: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Anton S Shabunin: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Natella I Enukashvily: Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia. ORCID
Olga V Supilnikova: Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia.
Anastasia I Konkina: Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia.
Natalia Yu Semenova: Research Department of Pathomorphology, Center for Preclinical and Translational Research, Federal State Budgetary Institution «Almazov National Medical Research Centre», Ministry of Health of Russia, 199034 St. Petersburg, Russia. ORCID
Gleb S Yatsemirsky: Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia.
Evgenii V Zinoviev: Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine, 192242 St. Petersburg, Russia. ORCID
Kristina N Rodionova: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Kirill L Kryshen: "Home of Pharmacy" Center, Leningrad Region, 188663 Kuzmolovsky, Russia.
Antonina Yu Borodina: "Home of Pharmacy" Center, Leningrad Region, 188663 Kuzmolovsky, Russia. ORCID
Alexander Yu Makarov: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Andrey M Fedyuk: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia.
Alexander D Nilov: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Elena V Chikulaeva: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia.
Lidiya S Konkova: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia.
Irina S Chustrak: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Veronika V Traxova: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia.
Platon A Safonov: Professor G.I. Gaivoronsky Laboratory of Experimental Traumatology and Orthopedics with Vivarium, H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia. ORCID
Sergey V Vissarionov: H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, 196603 St. Petersburg, Russia.
Egor M Prikhodko: Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia.
Yury V Yurkevich: Cell Technology Center Pokrovsky, 199066 St. Petersburg, Russia. ORCID
The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model. The rats (male Wistar, n = 100) were subjected to a full-thickness thermal burn (16 cm). Radical necrectomy was performed one day after the burn. Three days later, the rats were randomly assigned to one of four groups: group 1 (no treatment), group 2 (chloramphenicol and methyluracil ointment, a routine clinical treatment), group 3 (a gel without cells, mock treatment), and group 4 (a dermal fibroblast-impregnated HEC gel). The treatment lasted for five days. The wound-healing process was evaluated by planimetric, cytologic, histologic, and immunohistochemical methods. The differences in the rate of wound healing and the characteristics of wound cytology were identified. In the group 4, a regenerative type of cytogram was revealed, characterized by a significantly increased number of fibroblastic cells in comparison to samples from non-treated and mock-treated animals. Biopsy samples of burn wounds from animals in the group 4l demonstrated the presence of mature granulation tissue and a large number of microvessels. The repair process was stimulated, as evidenced by the increased thickness of newly formed granulation tissue and epidermis in the wound zone, elevated cellularity, and enhanced re-epithelialization activity. The number of Ki-67-positive proliferating cells was significantly higher in group 4 than in the control groups). A small number of non-proliferating donor fibroblasts was observed in the wound area 3 days after the end of treatment. The cell product is an effective agent for promoting wound healing during the regenerative phase. The experiments demonstrated that a gel populated by dermal fibroblasts can stimulate reparative regeneration processes in deep partial- and full-thickness burn wounds.
