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Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Nov, 2024;23 (11): 2029-2044.

Global transcriptional response of Pseudomonas aeruginosa to UVA radiation.

Martiniano M Ricardi, Paula M Tribelli, Cristina S Costa, Magdalena Pezzoni
Author Information
  1. Martiniano M Ricardi: IFIByNE (CONICET), Departamento de Fisiolog��a y Biolog��a Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
  2. Paula M Tribelli: IQUIBICEN (CONICET), Departamento de Qu��mica Biol��gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
  3. Cristina S Costa: Departamento de Radiobiolog��a, Comisi��n Nacional de Energ��a At��mica, Av. Gral. Paz 1499B1650KNA, General San Mart��n, Prov. de Buenos Aires, Argentina.
  4. Magdalena Pezzoni: Departamento de Radiobiolog��a, Comisi��n Nacional de Energ��a At��mica, Av. Gral. Paz 1499B1650KNA, General San Mart��n, Prov. de Buenos Aires, Argentina. pezzoni@cnea.gov.ar. ORCID
PMID: 39470974 DOI: 10.1007/s43630-024-00649-9

Abstract

Ultraviolet A (UVA) radiation is the major fraction of UV radiation reaching the Earth's surface. Its harmful effects on microorganisms, due mainly to oxidative damage, have been exploited for development of natural solar and commercial UVA-based disinfection methods. In this work, the global transcriptional response of Pseudomonas aeruginosa exposed to ultraviolet A (UVA) radiation was analyzed. To conduct this study, we analyzed the whole transcriptome of the PAO1 strain grown to logarithmic phase under sublethal doses of UVA or in the dark. We found that a total of 298 genes responded to UVA with a change of at least two-fold (5.36% of the total P. aeruginosa genome), and showed equal amount of induced and repressed genes. An important fraction of the induced genes were involved in the response to DNA damage and included induction of SOS, prophage and pyocins genes. The results presented in this study suggest that one of the main UVA targets are proteins carrying [Fe-S] clusters since several genes involved in the processes of synthesis, trafficking and assembly of these structures were upregulated. The management of intracellular iron levels also seems to be a robust response to this stress factor. The strong induction of genes involved in denitrification suggest that this pathway and/or reactive nitrogen species such as nitric oxide could have a role in the response to this radiation. Regarding the down-regulated genes, we found many involved in the biosynthesis of PQS, a quorum-sensing signal molecule with a possible role as endogenous photosensitizer.

Keywords

Pseudomonas aeruginosa Gene expression RNA-seq Transcriptome UVA Ultraviolet

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Grants

  1. PICT 2017-0598/Agencia Nacional de Promoci��n de la Investigaci��n, el Desarrollo Tecnol��gico y la Innovaci��n
  2. PIP 11220200100710/Consejo Nacional de Investigaciones Cient��ficas y T��cnicas

MeSH Term

Pseudomonas aeruginosa
Ultraviolet Rays
Transcription, Genetic
Gene Expression Regulation, Bacterial
DNA Damage
Transcriptome
Journal Article

Word Cloud

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