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International journal of pharmaceutics: X
Dec, 2024;8 100291.

Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: study on anti-bacterial activity, accelerate wound healing, and anti-inflammation.

Sirikwan Sangboonruang, Natthawat Semakul, Kiattikhun Manokruang, Nuttawut Khammata, Kanyaluck Jantakee, Katanchalee Mai-Ngam, Satrawut Charoenla, Phadungkiat Khamnoi, Kanokwan Saengsawang, Usanee Wattananandkul, Sorasak Intorasoot, Khajornsak Tragoolpua
Author Information
  1. Sirikwan Sangboonruang: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
  2. Natthawat Semakul: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  3. Kiattikhun Manokruang: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  4. Nuttawut Khammata: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  5. Kanyaluck Jantakee: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  6. Katanchalee Mai-Ngam: National Metal and Materials Technology Center, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
  7. Satrawut Charoenla: National Metal and Materials Technology Center, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
  8. Phadungkiat Khamnoi: Diagnostic Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  9. Kanokwan Saengsawang: Department of Medical Technology, Lampang Hospital, Lampang 52000, Thailand.
  10. Usanee Wattananandkul: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
  11. Sorasak Intorasoot: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
  12. Khajornsak Tragoolpua: Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
PMID: 39493006 DOI: 10.1016/j.ijpx.2024.100291

Abstract

Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-based therapy holds promise as a potential therapeutic approach to overcoming drug resistance. Conventional wound dressing is a passive strategy for wound care that is not capable of eradicating pathogens and promoting tissue repair. In this study, we aim to construct an advanced wound dressing; a thermo-responsive hydrogel incorporated with lactoferricin (Lfcin) niosome (Lfcin-Nio/hydrogel) for bacterial pathogen treatment. The Lfcin-loaded niosome (Lfcin-Nio) has a particle size of 396.91 ± 20.96 nm, 0.38 ± 0.01 of PdI, -10.5 ± 0.3 mV of ζ potential, and 72.30 ± 7.05 % Lfcin entrapment efficiency. Lfcin-Nio exhibited broad antibacterial activity on both drug-susceptible and drug-resistant strains, and also on bacteria residing in the biofilm matrix. The Lfcin-Nio/hydrogel was fabricated from 0.5 % / poloxamer 188-20 % w/v poloxamer 407, and supplemented with Lfcin-Nio and epidermal growth factor (EGF). The physical properties of Lfcin-Nio/hydrogels showed elasticity, swelling ability, and strong injectability with responsiveness to 33-37 °C temperatures. The biological properties of Lfcin-Nio/hydrogels exhibited a bactericidal effect against drug-resistant strains of and and showed less toxicity to the human skin fibroblast. It also promoted the healing of scratches by 55 % within 6 h, compared to the wound closure rate of 20 % in the cell control. The inflammatory response of the Lfcin-Nio/hydrogel-treated cells was reduced suppression of and mRNA expressions. From this study, Lfcin-Nio/hydrogels can be suggested as a modern wound dressing that possesses multifunctional and beneficial properties for the management of chronic wound infections.

Keywords

Anti-bacterial activity Anti-inflammation Lfcin-Nio Thermo-responsive hydrogel Wound healing

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