Excess free radicals can have some negative effects on human health. In this paper, a nanozyme was successfully constructed by the coordination of copper ions and tannic acid, and its structure and elemental distribution were determined by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy. Free radical scavenging experiments confirmed that it possessed superoxide dismutase-like activity, catalase-like activity, and hydroxyl radical scavenging ability. The results of thermogravimetric analysis experiments demonstrated that it possessed good thermal stability. A polyacrylonitrile hybrid nanofibrous membrane loaded with Cu/TA nanozyme was successfully constructed by electrospinning technology, and the maximum scavenging rate of DPPH and ABTS radicals can reach 64.22% and 58.44%, respectively. The nanofiber membrane also exhibited the ability to protect cells from oxidative stress damage. Therefore, the hybrid nanofibrous membrane has a broad application prospect in fields such as food preservation and biomedicine.
