Diversity of Improved Diploids and Commercial Triploids from spp. via Molecular Markers.
Juliana Rodrigues Sampaio, Wanderley Diaciso Dos Santos Oliveira, Luiz Carlos de Souza Junior, Fernanda Dos Santos Nascimento, Ricardo Franco Cunha Moreira, Andresa Priscila de Souza Ramos, Janay Almeida Dos Santos-Serejo, Edson Perito Amorim, Renata Darilia Moraes de Jesus, Claudia Fortes Ferreira
Author Information
Juliana Rodrigues Sampaio: Department of Agricultural, Environmental and Biological Sciencies, Federal University of Rec��ncavo da Bahia, Rua Rui Barbosa, 710-Centro, Cruz das Almas 44380-000, BA, Brazil. ORCID
Wanderley Diaciso Dos Santos Oliveira: Department of Biological Sciences, Feira de Santana State University, Feira de Santana 44036-900, BA, Brazil. ORCID
Luiz Carlos de Souza Junior: Department of Agricultural, Environmental and Biological Sciencies, Federal University of Rec��ncavo da Bahia, Rua Rui Barbosa, 710-Centro, Cruz das Almas 44380-000, BA, Brazil. ORCID
Fernanda Dos Santos Nascimento: Embrapa Mandioca e Fruticultura, Rua Embrapa, s/no, Cruz das Almas 44380-000, BA, Brazil. ORCID
Ricardo Franco Cunha Moreira: Department of Agricultural, Environmental and Biological Sciencies, Federal University of Rec��ncavo da Bahia, Rua Rui Barbosa, 710-Centro, Cruz das Almas 44380-000, BA, Brazil. ORCID
Andresa Priscila de Souza Ramos: Embrapa Mandioca e Fruticultura, Rua Embrapa, s/no, Cruz das Almas 44380-000, BA, Brazil. ORCID
Janay Almeida Dos Santos-Serejo: Embrapa Mandioca e Fruticultura, Rua Embrapa, s/no, Cruz das Almas 44380-000, BA, Brazil. ORCID
Edson Perito Amorim: Embrapa Mandioca e Fruticultura, Rua Embrapa, s/no, Cruz das Almas 44380-000, BA, Brazil. ORCID
Renata Darilia Moraes de Jesus: Department of Agricultural, Environmental and Biological Sciencies, Federal University of Rec��ncavo da Bahia, Rua Rui Barbosa, 710-Centro, Cruz das Almas 44380-000, BA, Brazil.
Claudia Fortes Ferreira: Embrapa Mandioca e Fruticultura, Rua Embrapa, s/no, Cruz das Almas 44380-000, BA, Brazil. ORCID
Banana breeding consists of obtaining diploid, triploid, and tetraploid intra- and interspecific hybrids by conventional breeding methods with the objective of aggregating characteristics of agronomic and commercial interest. Given the narrow genetic base of bananas, Embrapa's Banana Genetic Breeding Program (BGBP) aims at crosses between improved diploids (ID) (ID �� ID) and between improved diploids (ID) and commercial triploids (ID �� CTP) and tetraploids (ID �� CTT), in order to increase the genetic base and variability in bananas regarding agronomic traits of interest and resistance to main biotic and abiotic factors. These improved diploids are resistant to main fungal diseases such as yellow (YSD) and black Sigatoka (BSD) disease and Fusarium wilt (race 1 and subtropical race 4), the latter being one of the most devastating diseases in bananas. The genetic diversity between 22 improved diploids and seven commercial banana triploids was analyzed using DNA molecular markers. Five IRAP (Inter-Retrotransposon Amplified Polymorphism, 7 ISSR (Inter-Simple Sequence Repeats) and 12 SSR (Simple Sequence Repeat) markers were used. The genetic dissimilarity matrix was based on the Jaccard dissimilarity index; clusters were separated using the UPGMA (Unweighted Pair Group Method With Arithmetic Mean) method and cophenetic correlation of 0.8755. This study of the genetic diversity between improved diploids and commercial triploids, based on the genetic dissimilarity matrix, revealed that the most dissimilar diploids were DM23 and DM15 (74%) and DM16 and DM15 (74%). The smallest genetic distances between the improved diploids and commercial triploids were between TCGN25 and DM17 (50%) and TCN26 and DM17 (50%). The genetic distance matrix also revealed important genotypes to be used in crosses in order to maintain good characteristics in commercial triploids when crossed with improved diploids. The results of our study provide better breeding strategies for one of the largest banana-breeding programs worldwide focused on the development of banana varieties resistant to main biotic and abiotic factors.
