Sergio Liga: Department of Applied Chemistry and Engineering of Organic and Natural Compounds, Faculty of Chemical Engineering, Biotechnologies and Environmental Protection, Politehnica University Timisoara, Vasile P��rvan No. 6, 300223 Timisoara, Romania. ORCID
Raluca Vod��: Department of Applied Chemistry and Environmental Engineering and Inorganic Compounds, Faculty of Chemical Engineering, Biotechnologies and Environmental Protection, Politehnica University Timisoara, Vasile P��rvan No. 6, 300223 Timisoara, Romania.
Lavinia Lupa: Department of Applied Chemistry and Environmental Engineering and Inorganic Compounds, Faculty of Chemical Engineering, Biotechnologies and Environmental Protection, Politehnica University Timisoara, Vasile P��rvan No. 6, 300223 Timisoara, Romania. ORCID
Cristina Paul: Department of Applied Chemistry and Engineering of Organic and Natural Compounds, Faculty of Chemical Engineering, Biotechnologies and Environmental Protection, Politehnica University Timisoara, Vasile P��rvan No. 6, 300223 Timisoara, Romania. ORCID
Nicoleta Sorina Neme��: Renewable Energy Research Institute-ICER, Politehnica University Timisoara, Gavril Musicescu Street No. 138, 300501 Timisoara, Romania. ORCID
Delia Muntean: Multidisciplinary Research Center on Antimicrobial Resistance, Department of Microbiology, Faculty of Medicine, "Victor Babe��" University of Medicine and Pharmacy, 2nd Eftimie Murgu Sq., 300041 Timisoara, Romania. ORCID
��tefana Avram: Department of Pharmacognosy, Faculty of Pharmacy, "Victor Babe��" University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. ORCID
Mihaela Gherban: National Institute for Research and Development in Electrochemistry and Condensed Matter, P. Andronescu Street, No. 1, 300224 Timisoara, Romania. ORCID
Francisc P��ter: Department of Applied Chemistry and Engineering of Organic and Natural Compounds, Faculty of Chemical Engineering, Biotechnologies and Environmental Protection, Politehnica University Timisoara, Vasile P��rvan No. 6, 300223 Timisoara, Romania. ORCID
BACKGROUND: Zinc Oxide nanobiocomposites were successfully synthesized using a green synthesis approach. The process involves the utilization of the isoflavonepuerarin, resulting in the formation of PUE-ZnO NPs. METHODS: Physico-chemical and biological characterization techniques including X-ray dif-fraction (XRD), UV-vis spectroscopy, Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), and in ovo methods were employed to study the main characteristics of this novel hybrid material. RESULTS: The PUE-ZnO NPs were confirmed to have been successfully synthesized with a UV absorption peak at 340 nm, the XRD analysis demonstrating their high purity and crystallinity. The energy band-gap value of 3.30 eV suggests possible photocatalytic properties. Both SEM and AFM images revealed the nanoparticle`s quasi-spherical shape, roughness, and size. Good tolerability and anti-irritative effects were recorded in ovo on the chorioallantoic membrane (CAM). CONCLUSIONS: According to these results, the synthesis of green PUE-ZnO NPs may be a promising future approach for biomedical and personal care applications.