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Molecules (Basel, Switzerland)
Nov 20, 2024;29 (22):

Single-Component Starch-Based Hydrogels for Therapeutic Delivery.

Alfio Pulvirenti, Antonella Caterina Boccia, Carolina Constantin, Mihaela Surcel, Adriana Munteanu, Victor-Eduard Peteu, Monica Neagu
Author Information
  1. Alfio Pulvirenti: Istituto di Scienze e Tecnologie Chimiche (SCITEC) "Giulio Natta", C.N.R., Via Alfonso Corti 12, 20133 Milano, Italy. ORCID
  2. Antonella Caterina Boccia: Istituto di Scienze e Tecnologie Chimiche (SCITEC) "Giulio Natta", C.N.R., Via Alfonso Corti 12, 20133 Milano, Italy. ORCID
  3. Carolina Constantin: "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania. ORCID
  4. Mihaela Surcel: "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania. ORCID
  5. Adriana Munteanu: "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania. ORCID
  6. Victor-Eduard Peteu: "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania. ORCID
  7. Monica Neagu: "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independenței, 050096 Bucharest, Romania. ORCID
PMID: 39598852 DOI: 10.3390/molecules29225463

Abstract

Hydrogels are interesting materials as delivery systems of various therapeutic agents, mainly due to the water-swollen network and the localized and sustained drug release. Herein, single-component starch-based hydrogels with enhanced degradation rates were produced by applying a facile synthesis and proposed for a novel delivery system of therapeutic molecules. Starch was oxidized with sodium periodate in water and mild conditions to generate aldehyde derivatives that, after a freeze-thaw procedure, were allowed to compact and stable hydrogels. Oxidized starch was also cross-linked with asparagine through a Schiff base reaction to link the active molecule directly to the polysaccharide structure. The materials were structurally and morphologically characterized, and the ability to adsorb and release over time an active molecule was proven by qNMR spectroscopy. The cytotoxicity was evaluated on CAL-27 cell line (oral squamous cell carcinoma). Results indicated that synthesized hydrogels lead to a "frozen proliferative" state on cells due to the swelling capability in the cell medium. This behavior was confirmed by flow cytometry data indicating the hydrogels induced less "early apoptosis" and more "late apoptosis" in the cells, compared to the untreated control. Since the proposed materials are able to control the cell proliferation, they could open a new scenario within the field of precise therapeutic applications.

Keywords

NMR cytotoxicity flow cytometry hydrogels starch therapeutic delivery

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MeSH Term

Hydrogels
Starch
Humans
Cell Line, Tumor
Drug Delivery Systems
Cell Proliferation
Apoptosis
Drug Liberation
Drug Carriers
Cell Survival

Chemicals

Hydrogels
Starch
Drug Carriers
Journal Article

Word Cloud

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