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Yonsei medical journal
Dec, 2024;65 (12): 727-740.

Effects of LM1215 on and .

Won-Young Bae, Young Jin Lee, Subin Jo, So Lim Shin, Tae-Rahk Kim, Minn Sohn, Hyun-Joo Seol
Author Information
  1. Won-Young Bae: Microbiome R&D Center, Lactomason, Seoul, Korea. ORCID
  2. Young Jin Lee: Microbiome R&D Center, Lactomason, Seoul, Korea. ORCID
  3. Subin Jo: Center for Research and Development, Lactomason, Jinju, Korea. ORCID
  4. So Lim Shin: Microbiome R&D Center, Lactomason, Seoul, Korea. ORCID
  5. Tae-Rahk Kim: Microbiome R&D Center, Lactomason, Seoul, Korea. ORCID
  6. Minn Sohn: Microbiome R&D Center, Lactomason, Seoul, Korea. ORCID
  7. Hyun-Joo Seol: Department of Obstetrics and Gynecology, Kyung Hee University Hospital at Gangdong, Seoul, Korea. seolhj@khu.ac.kr. ORCID
PMID: 39609088 DOI: 10.3349/ymj.2023.0490

Abstract

PURPOSE: The aim of this study was to identify novel vaginal probiotics with the potential to prevent vulvovaginal candidiasis (VVC) and bacterial vaginosis (BV).
MATERIALS AND METHODS: Eighteen strains of were isolated from healthy Korean women, and their antimicrobial effects against and were assessed. Three strains ( LM1203, LM1209, and LM1215) were selected for further investigation, focusing on their growth inhibition, biofilm regulation, and cellular mechanisms against these vaginal pathogens. Additionally, electron microscopy revealed damage to induced by LM1215, and genomic analysis was conducted on this strain.
RESULTS: LM1203, LM1209, and LM1215 showed approximately 1 and 2 Log CFU/mL growth reduction in and , respectively. These strains effectively inhibited biofilm formation and eliminated the mature biofilms formed by . Furthermore, LM1215 decreased tricarboxylic acid cycle activity by 51.75 (<0.001) and respiratory metabolic activity by 52.88% (<0.001) in . induced cellular membrane damage, inhibition of protein synthesis, and cell wall collapse in . Genomic analysis confirmed LM1215 as a safe strain for vaginal probiotics.
CONCLUSION: The LM1215 is considered a safe probiotic agent suitable for the prevention of VVC and BV.

Keywords

Lactiplantibacillus plantarum bacterial vaginosis microbial sensitivity test probiotics vulvovaginal candidiasis

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Grants

  1. 121026-3/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries

MeSH Term

Candida albicans
Gardnerella vaginalis
Humans
Female
Biofilms
Probiotics
Candidiasis, Vulvovaginal
Lactobacillus plantarum
Vaginosis, Bacterial
Vagina
Journal Article

Word Cloud

Created with Highcharts 10.0.0LM1215vaginalprobioticsstrainsvulvovaginalcandidiasisVVCbacterialvaginosisBVLM1203LM1209growthinhibitionbiofilmcellulardamageinducedanalysisstrainactivity<0001safePURPOSE:aimstudyidentifynovelpotentialpreventMATERIALSANDMETHODS:EighteenisolatedhealthyKoreanwomenantimicrobialeffectsassessedThreeselectedinvestigationfocusingregulationmechanismspathogensAdditionallyelectronmicroscopyrevealedgenomicconductedRESULTS:showedapproximately12LogCFU/mLreductionrespectivelyeffectivelyinhibitedformationeliminatedmaturebiofilmsformedFurthermoredecreasedtricarboxylicacidcycle5175respiratorymetabolic5288%membraneproteinsynthesiscellwallcollapseGenomicconfirmedCONCLUSION:consideredprobioticagentsuitablepreventionEffectsLactiplantibacillusplantarummicrobialsensitivitytest

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