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Journal of water and health
Nov, 2024;22 (11): 2194-2205.

First report on the long-term viability of species in unpreserved environmental freshwater samples stored at room temperature.

Frederick R Masangkay, Fausto C Rona Iv, Angel Jasmine G Poricallan, Raphael Louis M Padua, Marielle Sabina S Reyes, Justine Kate M Ricacho, Monica Jane E Samar, Maria Carmela I Santos, Jitbanjong Tangpong, Manas Kotepui, Mark F F E Padua, Giovanni D Milanez, Panagiotis Karanis
Author Information
  1. Frederick R Masangkay: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines E-mail: frmasangkay@ust.edu.ph. ORCID
  2. Fausto C Rona Iv: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  3. Angel Jasmine G Poricallan: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  4. Raphael Louis M Padua: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  5. Marielle Sabina S Reyes: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  6. Justine Kate M Ricacho: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  7. Monica Jane E Samar: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  8. Maria Carmela I Santos: Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines.
  9. Jitbanjong Tangpong: Biomedical Sciences Program, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
  10. Manas Kotepui: Medical Technology, Faculty of Science, Nakhon Phanom University, Nakhon Phanom 48000, Thailand.
  11. Mark F F E Padua: The Manila Times Colleges of Subic, Subic Bay Freeport Zone 2222, Zambales, Philippines.
  12. Giovanni D Milanez: Department of Medical Technology, South SEED LPDH College, Las Piñas, 1740, Philippines.
  13. Panagiotis Karanis: Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne 50923, Germany; Medical School, Department of Basic and Clinical Science, University of Nicosia, Nicosia 2417, Cyprus. ORCID
PMID: 39611678 DOI: 10.2166/wh.2024.391

Abstract

is an opportunistic, free-living amoeba ubiquitous in the environment. Despite reports of its wide distribution in the Philippines' freshwater resources, more information on the long-term viability of the species is needed. This study aimed to define the long-term viability of species in unpreserved environmental freshwater samples after 3 years of storage at room temperature. Stored water samples from 15 study sites were filtered through a 1.2-μm pore size glass microfiber filter, cultured in non-nutrient agar (NNA) lawned with , and observed for amoebic growth for 14 days using light microscopy. Isolates from positive NNA culture were subjected to polymerase chain reaction (PCR) using JDP1 and JDP2 -specific primers. The study site positivity was 33% (5/15). genotype T4 and were isolated from Luzon; was isolated from Visayas; and sp. and genotype T20 were isolated from Mindanao. The long-term viability of species is an added risk factor for the sustained contamination of aquatic resources and other sample matrices. This heightens the risk of transmission to humans and animals. This study demonstrated that water samples fated for studies can be stored unpreserved at room temperature for several years.

Keywords

amoeba cyst parasite protozoa trophozoite water

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MeSH Term

Acanthamoeba
Fresh Water
Temperature
Philippines
Specimen Handling
Journal Article

Word Cloud

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