In silico and in vitro evaluation of potential agonistic and antagonistic estrogenic and androgenic activities of pure cyanotoxins, microcystin-LR and cylindrospermopsin.
Antonio Casas-Rodr��guez, Antonio Cascajosa-Lira, Mar��a Puerto, Ana Mar��a Came��n, Angeles Jos
Author Information
Antonio Casas-Rodr��guez: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor Garc��a Gonz��lez n�� 2, Sevilla 41012, Spain.
Antonio Cascajosa-Lira: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor Garc��a Gonz��lez n�� 2, Sevilla 41012, Spain.
Mar��a Puerto: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor Garc��a Gonz��lez n�� 2, Sevilla 41012, Spain. Electronic address: mariapuerto@us.es.
Ana Mar��a Came��n: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor Garc��a Gonz��lez n�� 2, Sevilla 41012, Spain.
Angeles Jos: Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor Garc��a Gonz��lez n�� 2, Sevilla 41012, Spain.
The potential endocrine disruption activity of cyanotoxins, particularly their effects on estrogen and androgen receptors (ER, AR), remains poorly understood. In the present study, the potential agonistic/antagonistic estrogenic and androgenic activities of MC-LR and CYN have been determined for the first time with validated OECD Test Guidelines No. 455 and 458, respectively. The data show that only MC-LR demonstrated weak estrogenic agonistic effects (LogPC value of -9.85���M), while both toxins displayed antagonistic effects on the ER, with LogIC values of -4.4 and -6.4 for MC-LR and CYN, respectively. In addition, neither MC-LR nor CYN exhibited agonistic/antagonistic activities in AR. Docking studies revealed potential interactions between both toxins and AR, with CYN showing a higher predicted affinity for this receptor. In vivo studies, particularly those investigating androgen disruption, are warranted to confirm the endocrine disrupting potential of MC-LR and CYN.
