Ultraviolet germicidal irradiation (UVGI) technology can inhibit the environmental transmission of airborne pathogens, but the dose-response behavior of airborne human coronavirus and wavelength-specific inactivation mechanisms are not well understood. This study investigated three competitive UVC sources for their inactivation efficacy and mechanisms against human coronavirus OC43 (HCoV-OC43). Results showed the following order of inactivation efficacy: 222-nm KrCl excimer lamp > 263-nm UV-LEDs > 254-nm low-pressure mercury lamp. The 222-nm KrCl excimer lamp achieved a 5-log inactivation of aerosolized HCoV-OC43 with a dose of less than 1 mJ/cm��, while the 263-nm UV-LEDs had the highest genome damage rate constant at 7.08 �� 0.85 mJ/cm��. Although 222-nm Far-UVC caused less genome damage, it affected viral proteins more significantly, specifically the nucleocapsid (N) and spike (S) proteins, which lead to compromising capsid integrity and binding ability to host cells. Capsid integrity RT-qPCR and binding assay RT-qPCR used in this study could better monitor infectivity of airborne coronavirus than standard RT-qPCR. Additionally, significant lipid oxidation of HCoV-OC43 was observed under 222-nm irradiation, potentially impacting overall inactivation efficacy. This study provides detailed evidence on the effects of different UVC wavelengths on airborne HCoV-OC43, contributing to the optimization of UVC irradiation for indoor bioaerosol disinfection.
