Pengcheng Fu: Zhejiang Key Laboratory of Micro-nano Quantum Chips and Quantum Control, School of Physics, Zhejiang University, Hangzhou 310027, China. ORCID
Yongqing Zhang: Zhejiang Key Laboratory of Micro-nano Quantum Chips and Quantum Control, School of Physics, Zhejiang University, Hangzhou 310027, China. ORCID
Siming Wang: Zhejiang Key Laboratory of Micro-nano Quantum Chips and Quantum Control, School of Physics, Zhejiang University, Hangzhou 310027, China.
Xin Ye: Key Laboratory of Oral Biomedical Research of Zhejiang Province, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Cancer Center of Zhejiang University, Hangzhou 310006, China. ORCID
Yunhong Wu: Key Laboratory of Oral Biomedical Research of Zhejiang Province, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Cancer Center of Zhejiang University, Hangzhou 310006, China.
Mengfei Yu: Key Laboratory of Oral Biomedical Research of Zhejiang Province, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Cancer Center of Zhejiang University, Hangzhou 310006, China. ORCID
Shiyao Zhu: Zhejiang Key Laboratory of Micro-nano Quantum Chips and Quantum Control, School of Physics, Zhejiang University, Hangzhou 310027, China.
Hyeon Jeong Lee: College of Biomedical Engineering & Instrument Science, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China. ORCID
Delong Zhang: Zhejiang Key Laboratory of Micro-nano Quantum Chips and Quantum Control, School of Physics, Zhejiang University, Hangzhou 310027, China. ORCID
Molecular spectroscopy provides intrinsic contrast for in situ chemical imaging, linking the physiochemical properties of biomolecules to the functions of living systems. While stimulated Raman imaging has found successes in deciphering biological machinery, many vibrational modes are Raman inactive or weak, limiting the broader impact of the technique. It can potentially be mitigated by the spectral complementarity from infrared (IR) spectroscopy. However, the vastly different optical windows make it challenging to develop such a platform. Here, we introduce in situ pump-probe IR and Raman excitation (INSPIRE) microscopy, a nascent cross-modality spectroscopic imaging approach by encoding the ultrafast Raman and the IR photothermal relaxation into a single probe beam for simultaneous detection. INSPIRE inherits the merits of complementary modalities and demonstrates high-content molecular imaging of chemicals, cells, tissues, and organisms. Furthermore, INSPIRE applies to label-free and molecular tag imaging, offering possibilities for optical sensing and imaging in biomedicine and materials science.
