Effect of ozonation on the phytochemicals of black seed oil and its anti-microbial, anti-oxidant, anti-inflammatory, and anti-neoplastic activities in vitro.
Aisha M H Al-Rajhi, Tarek M Abdelghany, Mohammed S Almuhayawi, Mohammed H Alruhaili, Amna A Saddiq, Afra M Baghdadi, Soad K Al Jaouni, Hibah M Albasri, Moayad S Waznah, Faisal A Alraddadi, Samy Selim
Author Information
Aisha M H Al-Rajhi: Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Tarek M Abdelghany: Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11725, Egypt. tabdelghany.201@azhar.edu.eg.
Mohammed S Almuhayawi: Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Mohammed H Alruhaili: Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Amna A Saddiq: Department of Biological Sciences, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
Afra M Baghdadi: Department of Biological Sciences, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
Soad K Al Jaouni: Department of Hematology/Oncology, Yousef Abdulatif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Hibah M Albasri: Department of Biology, College of Science, Taibah University, Madinah, Saudi Arabia.
Moayad S Waznah: Department of Biology, College of Science, Taibah University, Madinah, Saudi Arabia.
Faisal A Alraddadi: Department of Biology, College of Science, Taibah University, Madinah, Saudi Arabia.
Samy Selim: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia. sabdulsalam@ju.edu.sa.
Black seed has been applied for several decades to cure an extensive variety of illnesses and ailments. In this report, the chemical profile of both crude and ozonized black seed oil was assessed after the oil was exposed to 0 to 5 L/minute of ozone for four hours. The in vitro effects of black seed oil following being exposed to ozone including antimicrobial properties versus Bacillus cereus (ATCC11778), Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8739), Salmonella typhi (ATCC 6539), and Klebsiella pneumoniae (ATCC13883), Candida albicans (ATCC10221), and Aspergillus niger (ATCC16888). Besides, antioxidant effects, anti-inflammatory capacity, and antineoplastic function versus HCT cells were assessed. The chemical profile of ozonized black seed oil showed elevation of essential molecules of oil as well as presence of some characteristic molecules to both forms of oil. Besides, it could be noticed that exposing of oil to ozone improves its antimicrobial activity towards all tested microbes except for C. albicans. Both forms of oil showed no activity towards A. niger. Black seed oil exposed to ozone showed a promising antioxidant capacity with IC of 2.93 ± 0.2 µg/ml. A dramatic improvement in anti-inflammatory impact of ozonized oil as well as its antitumor capacity towards HCT cells could be seen in the laboratory outcomes. The current findings point to a novel method for enhancing some of the in vitro medicinal uses of black seed oil by exposing it to ozone.
