Detection of a novel hepacivirus in wild cavies (Cavia aperea aperea).
Gabriela Espíndola Birlem, Alexandre Sita, Juliana Schons Gularte, Deivid de Souza da Silva, Meriane Demoliner, Paula Rodrigues de Almeida, Juliane Deise Fleck, Fernando Rosado Spilki, Severino Silvano Dos Santos Higino, Sergio Santos de Azevedo, Matheus Nunes Weber
Author Information
Gabriela Espíndola Birlem: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Alexandre Sita: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Juliana Schons Gularte: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Deivid de Souza da Silva: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Meriane Demoliner: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Paula Rodrigues de Almeida: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Juliane Deise Fleck: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Fernando Rosado Spilki: Laboratório de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo, RS, Brazil.
Severino Silvano Dos Santos Higino: Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande (UFCG), Patos, PB, Brazil.
Sergio Santos de Azevedo: Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande (UFCG), Patos, PB, Brazil.
Matheus Nunes Weber: Laboratório de Imunologia e Biologia Molecular, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. matheus.weber@ufrgs.br. ORCID
Hepacivirus is a genus of RNA viruses within the family Flaviviridae of which hepatitis C virus (HCV) is the prototype. Several hepaciviruses have been identified in mammals, including rodents of multiple families. Each rodent hepacivirus described so far has been found only in members of a single rodent species. Here, we report the discovery and characterization of a putative new genotype of an unclassified rodent hepacivirus in a wild cavy (Cavia aperea aperea) that was reported previously in Proechimys semispinosus. This virus was detected in one out of 14 (7.14%) wild cavy sera tested by RT-PCR. The complete genome sequence was obtained by high-throughput sequencing using an Illumina MiSeq platform. This is the first report of a hepacivirus in a member of the family Caviidae. Our findings show that members of different rodent species and even families can be infected by hepaciviruses of the same species. The identification and characterization of novel hepaciviruses might lead to the discovery of reservoirs of viruses that are genetically related to human pathogens, and this can help to elucidate the evolutionary origins of HCV and other hepaciviruses.
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