Phytochemical Analysis and Antidiarrheal Activity of Stem Bark Decoctions of Sabine (Clusiaceae).
Ericka Lorleil Mayindza Ekaghba, Manon Grenet, Pierrick Gandolfo, Corinne Loutelier-Bourhis, Isabelle Schmitz, Carlos Afonso, Patrice Lerouge, Line Edwige Mengome
Author Information
Ericka Lorleil Mayindza Ekaghba: Institut de Pharmacopée et Médecines Traditionnelles (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Libreville BP 12 141, Gabon.
Manon Grenet: Université de Rouen Normandie (UNIROUEN), Normandie Univeristy, Inserm U1245, F-76000 Rouen, France.
Pierrick Gandolfo: Université de Rouen Normandie (UNIROUEN), Normandie Univeristy, Inserm U1245, F-76000 Rouen, France. ORCID
Corinne Loutelier-Bourhis: Université de Rouen Normandie, INSA Rouen, Normandie Univeristy, CNRS, UMR6014-COBRA, F-76000 Rouen, France. ORCID
Isabelle Schmitz: Université de Rouen Normandie, INSA Rouen, Normandie Univeristy, CNRS, UMR6014-COBRA, F-76000 Rouen, France. ORCID
Carlos Afonso: Université de Rouen Normandie, INSA Rouen, Normandie Univeristy, CNRS, UMR6014-COBRA, F-76000 Rouen, France. ORCID
Patrice Lerouge: Université de Rouen Normandie (UNIROUEN), Normandie Univeristy, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, F-76000 Rouen, France.
Line Edwige Mengome: Institut de Pharmacopée et Médecines Traditionnelles (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Libreville BP 12 141, Gabon.
is a medicinal plant of which bark decoctions are used in traditional medicine for the treatment of diarrhea symptoms in Gabon. The aim of the present work was to perform phytochemical and pharmacological analyses of decoctions of bark. In a principal approach, spectrophotometric analyses were used to quantify phenolic compounds, followed by liquid chromatography coupled to mass spectrometry analysis that allowed the identification of flavanone-flavone dimers as the main metabolites. Pharmacological analyses showed the absence of toxicity, thus confirming the safety of use of this decoction in traditional medicine. The antioxidant activity of the bark decoctions was demonstrated to depend on their phenolic contents. The decoction of stem barks harvested during the rainy season also induced a dose-dependent relaxation of isolated ileum fragments from Wistar rats. In addition, the antidiarrheal activity of barks was investigated against castor oil-induced diarrhea. The oral administration of different concentrations of this decoction led to a decrease in wet stools, indicating an antidiarrheal effect at the doses that were used. These results encourage the deepening of bio-guided research on bark decoctions in order to propose standard traditional medical treatments.
