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Arhiv za higijenu rada i toksikologiju
Dec 01, 2024;75 (4): 297-302.

An evaluation of the cytotoxic potential of medicinal mushrooms against human breast cancer cell lines.

Dijana Topalović, Lada Živković, Sunčica Borozan, Juan F Santibanez, Biljana Spremo-Potparević
Author Information
  1. Dijana Topalović: 1University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia. ORCID
  2. Lada Živković: 1University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia.
  3. Sunčica Borozan: 2University of Belgrade Faculty of Veterinary Medicine, Department of Chemistry, Belgrade, Serbia.
  4. Juan F Santibanez: 3University of Belgrade Institute for Medical Research National Institute of the Republic of Serbia, Department of Molecular Oncology, Belgrade, Serbia.
  5. Biljana Spremo-Potparević: 1University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia.
PMID: 39718091 DOI: 10.2478/aiht-2024-75-3915

Abstract

Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts auct. non Murrill (AB), (Berk.) Sacc. (CS), and Immune Assist (IA) have already been documented. This study, studied the influence of these three mushrooms on the viability of cell lines MCF-7, MDA-MB-231, and HS-5. The cytotoxicity of AB, CS, and IA at different concentrations (25, 50, 100, 200, 400 and 800 μg/mL) was evaluated using the MTT assay. The results showed that AB was the most effective and induced cytotoxicity in both cancer cell lines, with IC values of 96.7 μg/mL for MCF-7 and 368.4 μg/mL for MDA-MB-231. After treatment with CS and IA, the half-maximal inhibitory concentration was reached only in MDA- MB-231 cells (IC=613 μg/mL for CS and 343.3 μg/mL for IA). We have shown here that AB, CS and IA can suppress the growth of MCF-7 and MDA-MB-231 cell lines, while affecting the survival of healthy HS-5 cells to a much lesser extent. Our results suggested that AB, CS and IA are promising natural sources with potential anticancer activity.

Keywords

Agaricus blazei Cordyceps sinensis Immune Assist MTT citotoksičnost cytotoxicity

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MeSH Term

Humans
Breast Neoplasms
Female
Cell Line, Tumor
Agaricales
Agaricus
MCF-7 Cells
Cordyceps
Cell Survival
Antineoplastic Agents

Chemicals

Antineoplastic Agents
Journal Article

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