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Journal of clinical laboratory analysis
Dec 27, 2024; e25144.

Construction of Candida albicans pRB895-SAP2-SC5314 With SAP2 High Expression and Its Effects on Adhesion.

Lan Xue, Lu Yang, Bingqian Zhao, Wenli Feng, Jing Yang, Yan Ma
Author Information
  1. Lan Xue: The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
  2. Lu Yang: The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
  3. Bingqian Zhao: The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
  4. Wenli Feng: The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China. ORCID
  5. Jing Yang: The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
  6. Yan Ma: The Department of Dermatovenereology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
PMID: 39727206 DOI: 10.1002/jcla.25144

Abstract

BACKGROUND: SAP2 is closely associated with the pathogenicity and drug resistance of Candida albicans (C. albicans). Our study aimed to construct C. albicans with SAP2 overexpression (pRB895-SAP2-SC5314) to explore the influence of SAP2 on the adhesion of C. albicans and predict the interaction between magnolol and Sap2 by molecular docking.
METHODS: The recombinant plasmid pRB895-SAP2 with high SAP2 expression was prepared using a plasmid extraction kit and transformed into C. albicans strain SC5314 using an improved lithium acetate conversion method to construct PRB895-SAP2-SC5314. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to detect the expression of adhesion-related genes in the different strains. Molecular docking and visual analysis of magnolol and Sap2 were performed using the CB-DOCK2 platform.
RESULTS: Compared with the control SC5341 and SC5341 transfected with pRB895, SAP2 expression was significantly higher in the pRB895-SAP2-SC5314 strain (p < 0.05). Based on the sequencing and mapping results, the pRB895-SAP2-SC5314 strain was successfully prepared. SAP2 overexpression significantly downregulated ALS1 expression (p < 0.05), whereas ALS3, TEC1, HOG1, PHR1, and TUP1 expression was downregulated in C. albicans (p < 0.05). The optimal docking result for magnolol and Sap2 protein was -8.1 kcal/mol of vina score, which was considered good docking.
CONCLUSIONS: SAP2 overexpression may strengthen the adhesion and pathogenicity of C. albicans, and magnolol may act as an Sap2 inhibitor that affects the adhesion of C. albicans.

Keywords

C. albicans SAP2 SAP2adhesion magnolol pRB895‐SAP2‐SC5314 strain

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Grants

  1. 82072262/General Project of the National Natural Science Foundation of China
Journal Article

Word Cloud

Created with Highcharts 10.0.0albicansSAP2CmagnololexpressionpRB895-SAP2-SC5314Sap2dockingstrainoverexpressionadhesionusingp < 005pathogenicityCandidaconstructplasmidpreparedperformedSC5341significantlydownregulatedmayBACKGROUND:closelyassociateddrugresistancestudyaimedexploreinfluencepredictinteractionmolecularMETHODS:recombinantpRB895-SAP2highextractionkittransformedSC5314improvedlithiumacetateconversionmethodPRB895-SAP2-SC5314QuantitativereversetranscriptionpolymerasechainreactionqRT-PCRdetectadhesion-relatedgenesdifferentstrainsMolecularvisualanalysisCB-DOCK2platformRESULTS:ComparedcontroltransfectedpRB895higherBasedsequencingmappingresultssuccessfullyALS1whereasALS3TEC1HOG1PHR1TUP1optimalresultprotein-81 kcal/molvinascoreconsideredgoodCONCLUSIONS:strengthenactinhibitoraffectsConstructionHighExpressionEffectsAdhesionSAP2adhesionpRB895‐SAP2‐SC5314

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