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Food and environmental virology
Jan 04, 2025;17 (1): 11.

Detection of Rocahepevirus ratti in Bivalve Mollusks from S��o Lu��s Island, Maranh��o, Brazil: A Potential Transmission Route of an Emerging Zoonotic Pathogen?

Andreza Soriano Figueiredo, Isabella Rodrigues Negreiros, Aldaleia do Nascimento E Silva, Caroline Roberta Soares Salgado, Nat��lia Louren��o Dos Santos, Marcelo Alves Pinto, Alcina Vieira de Carvalho Neta, Jos�� Paulo Gagliardi Leite, Carina Pacheco Cantelli
Author Information
  1. Andreza Soriano Figueiredo: Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil. andrezasofi@gmail.com.
  2. Isabella Rodrigues Negreiros: Program in Animal Science, State of Maranh��o University, S��o Lu��s, Maranh��o, Brazil.
  3. Aldaleia do Nascimento E Silva: Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
  4. Caroline Roberta Soares Salgado: Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
  5. Nat��lia Louren��o Dos Santos: Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
  6. Marcelo Alves Pinto: Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil.
  7. Alcina Vieira de Carvalho Neta: Program in Animal Science, State of Maranh��o University, S��o Lu��s, Maranh��o, Brazil.
  8. Jos�� Paulo Gagliardi Leite: Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
  9. Carina Pacheco Cantelli: Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
PMID: 39754637 DOI: 10.1007/s12560-024-09624-0

Abstract

The attempt to investigate hepatitis E virus (HEV) contamination in naturally growing mangrove bivalve mollusks captured for local sale in a touristic area of Maranh��o state in Brazil revealed the detection of rat hepatitis E virus (ratHEV). Using international standard protocols for processing and nucleic acid extraction, we analyzed 89 bivalve samples (Mytella falcata and Crassostrea rhizophorae) with two broadly reactive assays: heminested pan-Hepeviridae (ORF-1) and probe-based HEV-1 to HEV-4 (ORF-2/ORF-3). Heminested reactions presented 2 (2.2%) amplifications of the expected size. Nucleotide identities ranged from 86.6 to 89.0% with ratHEV isolates from wild rats, pigs, one human case and sewage reported in different countries. Regarding the phylogenetic tree, the sequences grouped with genotype HEV-C1. This first report of ratHEV detection in bivalve mollusks may be a starting point for further research on virus variability, distribution, host range and especially the possible role of contaminated shellfish as a vehicle for ratHEV transmission.

Keywords

Rocahepevirus ratti Bivalve mollusk Food safety HEV-C1 Hepatitis E virus Zoonosis

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Grants

  1. 402639/2023-5/The Brazilian National Council for Scientific and Technological Development (CNPq)
  2. 402987/2023-3/The Brazilian National Council for Scientific and Technological Development (CNPq)
  3. FAPERJ E-26/210.045/2023/Funda����o Carlos Chagas Filho de Amparo �� Pesquisa do Estado do Rio de Janeiro
  4. FAPERJ/SEI-260003/001753/2023/Funda����o Carlos Chagas Filho de Amparo �� Pesquisa do Estado do Rio de Janeiro
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