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International journal of molecular sciences
Dec 19, 2024;25 (24):

Genome-Wide Identification and Analysis of Transcription Factor Family Genes Involved in Cold Stress Tolerance in Winter Rapeseed ( L.).

Yanxia Xu, Li Ma, Xiucun Zeng, Yaozhao Xu, Xiaolei Tao, Abbas Muhammad Fahim, Lijun Liu, Junyan Wu, Gang Yang, Yuanyuan Pu, Tingting Fan, Wangtian Wang, Wancang Sun
Author Information
  1. Yanxia Xu: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  2. Li Ma: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China. ORCID
  3. Xiucun Zeng: College of Life Sciences and Engineering, Hexi University, Zhangye 734000, China.
  4. Yaozhao Xu: College of Life Sciences and Engineering, Hexi University, Zhangye 734000, China.
  5. Xiaolei Tao: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  6. Abbas Muhammad Fahim: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China. ORCID
  7. Lijun Liu: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  8. Junyan Wu: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  9. Gang Yang: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  10. Yuanyuan Pu: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  11. Tingting Fan: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  12. Wangtian Wang: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
  13. Wancang Sun: State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
PMID: 39769355 DOI: 10.3390/ijms252413592

Abstract

TCP transcription factors are important during plant growth and stress responses. However, their role in the cold stress response of L. remains poorly understood. In this research, we identified the gene family in to learn the features of the gene family, functionally annotating the interacting proteins of TCP4 and analyzing their expression levels. Our results illustrated the presence of 19 members of the family in , exhibiting molecular weights ranging from 27,367.45 to 59,433.64 Da. All identified proteins were classified as unstable, with isoelectric points ranging from 5.5 to 9.48. Subcellular localization forecasted that TCP proteins were all positioned in the nucleus. The gene structure is relatively simple, with only seven members possessing introns, and none of the members contain UTR regions. comprise hormone-, light-, and stress-responsive elements. We found that the frequency of photoresponsive elements was greatest in the promoter region, suggesting that genes are regulated by light signals and function synergistically with plant growth and development. In addition, five candidate interaction proteins of were identified using yeast two-hybrid screening. RNA-Seq and q-PCR analyses of the interacting genes revealed differential expression of family genes across various tissues following cold stress. Significant responses were observed under low-temperature stress, drought stress, and rehydration treatment, suggesting that these genes play crucial roles as regulators of the molecular network mechanisms responding to stress. This study enhances our understanding of the family and provides significant insights into the stress tolerance mechanisms of

Keywords

Brassica rapa TCP transcription factor abiotic stress

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MeSH Term

Brassica rapa
Transcription Factors
Plant Proteins
Cold-Shock Response
Gene Expression Regulation, Plant
Multigene Family
Phylogeny
Promoter Regions, Genetic

Chemicals

Transcription Factors
Plant Proteins
Journal Article

Word Cloud

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