Yue Deng, Wenxian Wu, Xiaoqing Huang, Xiaoxiang Yang, Yaoyin Yu, Zhongmei Zhang, Zijin Hu, Xiquan Zhou, Kang Zhou, Yong Liu, Lei Zhang
Rhizosphere microbiomes are constantly mobilized during plant-pathogen interactions, and this, in turn, affects their interactions. However, few studies have examined the activities of rhizosphere microbiomes in plants with different susceptibilities to soil-borne pathogens, especially those that cause clubroot disease. In this study, we compared the rhizosphere bacterial community in response to infection of among the four different clubroot susceptibility cultivars of oilseed rape (). Our results revealed obvious differences in the responses of rhizosphere bacterial community to the infection between the four cultivars of oilseed rape. Several bacterial genera that are associated with the nitrogen cycle, including , , , , , and , showed significantly different changes between susceptible and resistant cultivars in the presence of infection. Moreover, increased connectedness and robustness were exhibited in the rhizosphere bacterial community co-occurrence network in clubroot-susceptible cultivars that were infected with , while only slight changes were observed in clubroot-resistant cultivars. Metagenomic analysis of microbial metabolism also indicated differences in the rhizosphere bacterial community between susceptible and resistant cultivars that were infected with . Functional analysis of the nitrogen cycle showed that genes related to nitrification () were upregulated in susceptible cultivars, while genes related to assimilatory nitrate reduction (, , and ) were upregulated in resistant cultivars that were infected with . These findings indicate that the synthesis and assimilation process of NO content were promoted in susceptible and resistant cultivars, respectively. Our study revealed differences in the characteristics of rhizosphere bacterial communities in response to infection between clubroot-susceptible and clubroot-resistant cultivars as well as the potential impact of these differences on the plant- interaction.
