Zehuai Yu: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Yiying Qi: College of Agriculture, Fujian Agriculture and Forestry University, No.15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.
Yuxuan Wei: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Gui Zhuang: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Yihan Li: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Baiyu Wang: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Sehrish Akbar: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Yi Xu: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Xiuting Hua: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Qiutao Xu: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Zuhu Deng: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Jisen Zhang: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Yongji Huang: Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Geography and Oceanography, Minjiang University, Minhou District, Fuzhou 350108, China.
Fan Yu: Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, 100 University East Road, Nanning 530004, China.
Jiannan Zhou: Key Laboratory of Tropical Fruit Biology (Ministry of Agriculture), South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Mazhang District, Zhanjiang 524091, China.
Oligonucleotide (Oligo)-based fluorescence hybridization (FISH) represents a highly effective methodology for identifying plant chromosomes. Longan is a commercially significant fruit species, yet lacking basic chromosomal markers has hindered its cytogenetic research. In this study, we developed a cost-effective oligo-based system for distinguishing chromosomes of longan ( Lour., 2 = 2 = 30). For this system, each synthesized oligo contained two chromosome-specific sequences that spanned a distance of over 200 kb, and a PCR-based flexible amplification method coupled with nested primers was used for probe labeling. The use of these oligo-based barcodes enabled the marking of 36 chromosomal regions, which allowed for the unambiguous distinction of all 15 chromosomes in both longan and lychee ( Sonn., 2 = 2 = 30) species. Based on the identification of individual chromosomes, we constructed karyotypes and detected genome assembly errors involving the 35S ribosomal RNA gene (35S rDNA) in longan and lychee. Developing oligo-based barcodes offers considerable promise for advancing cytogenetic research in longan, lychee, and their related species. Furthermore, this cost-effective synthesis system can be referred to the development of new oligo libraries among other species.
References
Gigascience. 2017 May 1;6(5):1-14
[PMID: 28368449]
Bioinformatics. 2003 Jan 22;19(2):301-2
[PMID: 12538260]
