Yun Long: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Zhi Li: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Menglu Li: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Peiyi Lu: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Yujia Deng: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Pengyao Wu: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Xue Li: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Gangjian Qin: Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China.
Jiamin Huang: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Wenying Gao: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Guobao Li: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Tianyuan Jia: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China.
Liang Yang: Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, China. ORCID
and are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential impacts on infections are largely unexplored. In this study, we first demonstrated that inhibits the growth of by iron chelating via quorum sensing. Next, through proteomic analysis, we discovered that the PQS molecule significantly changed a large amount of protein expression in , including proteins involved in the type VII secretion system and iron homeostasis. Furthermore, we revealed that PQS significantly enhanced the production of bacterial membrane vesicles (MVs) by . Our study suggests that the PQS can serve as an interspecies signaling molecule to communicate with and affect their physiology and virulence.
