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Diagnostics (Basel, Switzerland)
Feb 12, 2025;15 (4):

Distinct Phenotypic and Molecular Characteristics of CD34 and CD34 Hematopoietic Stem/Progenitor Cell Subsets in Cord Blood and Bone Marrow Samples: Implications for Clinical Applications.

Ameera Gaafar, Fatheia Nabeil Hamza, Rama Yousif, Zakia Shinwari, Aminah Ghazi Alotaibi, Alia Iqniebi, Khalid Al-Hussein, Amer Al-Mazrou, Pulicat Subramanian Manogaran, Tusneem Elhassan, Marcela Marquez-Méndez, Mahmood Aljurf, Hind Al-Humaidan, Ayodele Alaiya
Author Information
  1. Ameera Gaafar: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  2. Fatheia Nabeil Hamza: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia. ORCID
  3. Rama Yousif: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  4. Zakia Shinwari: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia. ORCID
  5. Aminah Ghazi Alotaibi: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  6. Alia Iqniebi: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  7. Khalid Al-Hussein: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  8. Amer Al-Mazrou: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  9. Pulicat Subramanian Manogaran: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  10. Tusneem Elhassan: Biochemistry and Molecular Medicine Department, Alfaisal University, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  11. Marcela Marquez-Méndez: Medicine Faculty, Universidad Autonoma de Nuevo Leon, Mitras Centro, Monterrey 64460, Mexico.
  12. Mahmood Aljurf: Cancer Center for Excellence, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia.
  13. Hind Al-Humaidan: Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
  14. Ayodele Alaiya: Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, P.O. Box 3354, Riyadh 11211, Saudi Arabia. ORCID
PMID: 40002599 DOI: 10.3390/diagnostics15040447

Abstract

We aimed to identify the molecular signatures of primitive CD34 and CD34 hematopoietic stem/progenitor cell (HSC/HPC) subsets in cord blood and bone marrow samples. CD34 and CD34 HSC/HPC subsets from cord blood and bone marrow were characterized using flow cytometry, real-time PCR, and proteomic analysis to evaluate their phenotypic and molecular profiles. Our findings revealed a significantly higher percentage of LinCD34CD38 (-/-) cells than of LinCD34CD38 (+/-) cells in cord blood. Aldehyde dehydrogenase levels were significantly lower in (-/-) than in (+/-) cells. Clonogenic ability was lower in (-/-) than in (+/-) cells. However, CD34 cells exhibited potent megakaryocyte/erythrocyte differentiation ability. Importantly, the HSC/HPC subsets expressed pluripotency or stemness genes (SOX2, Nanog, and OCT4); however, OCT4 expression significantly increased in (-/-) compared with (+/-) cells. We identified 304 proteins in the HSC/HPC subsets-85.6% had similar expression patterns in the two subsets; only 14.4% were differentially expressed between (-/-) and (+/-) cells. This implies their comparability at the protein level. Certain proteins were implicated in cellular-development-, gene-expression-, and embryonic-development-related signaling networks. Distinct biological and functional characteristics were observed between (-/-) and (+/-) HSC/HPC subsets. Some of the identified proteins may be novel HSC/HPC subsets markers for clinical applications after validation.

Keywords

CD34 HSC/HPC bone marrow cord blood hematopoietic proteomics and gene expression

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Grants

  1. grant number م ص-36-136/King Abdulaziz City for Science and Technology
Journal Article

Word Cloud

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