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Chembiochem : a European journal of chemical biology
Apr 01, 2025;26 (7): e202400879.

Nanopipettes for Chemical Analysis in Life Sciences.

Shiyu Zheng, Mansha Wu, Xiaoyuan Wang, Shuyue Xu, Ruocan Qian
Author Information
  1. Shiyu Zheng: Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R China.
  2. Mansha Wu: Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R China.
  3. Xiaoyuan Wang: Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R China.
  4. Shuyue Xu: Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R China.
  5. Ruocan Qian: Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Joint International Laboratory for Precision Chemistry, Frontiers Science Center for Materiobiology & Dynamic Chemistry School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P.R China. ORCID
PMID: 40014334 DOI: 10.1002/cbic.202400879

Abstract

Nanopipette-based assays have gained widespread applications in electrochemical and analytical technologies, achieving significant advancements over the past decade in DNA sequencing, biosensing, targeted delivery, and bioimaging. The ultrasmall tip size of nanopipettes bridges the gap between the macro- and nano worlds, which can be attributed to the capability of nanopipettes to transport ultrasmall volumes of liquids, ions, and solutes. In this review, we discuss the fabrication, characterization, and modification of nanopipettes to provide an overview of the recent developments of nanopipette-based sensors and strategies. We also introduce the recent studies developed by our group and other groups using nanopipettes for chemical analysis of life science. Finally, we discuss the future development of nanopipette-based strategies and their exciting potential for studying bioscience and biomedical engineering.

Keywords

electrochemistry fabrication modification methods nanopipettes sensors

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Grants

  1. 21977031/National Natural Science Foundation of China
  2. 22ZR1416800/Shanghai Science and Technology Committee
  3. 23ZR1416100/Shanghai Science and Technology Committee
  4. 222201717003/the Fundamental Research Funds for the Central Universities

MeSH Term

Biological Science Disciplines
Nanotechnology
Biosensing Techniques
Humans
Electrochemical Techniques
Nanostructures
Journal Article Review
Article has an altmetric score of 1

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