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Frontiers in plant science
2025;16 1533263.

Accumulation differences of high-value ingredients in different phenotype insights from integrative metabolome and transcriptome analyses.

Juan Zeng, Yu Qing Long, Jia Yuan Zhu, Xue Sen Fu, Jing Yu Zhang, Jia Wei He, Xiao Rong Liu, Zhi Hui Wang, Qiao Zhen Tong, Xiang Dan Liu, Ri Bao Zhou
Author Information
  1. Juan Zeng: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  2. Yu Qing Long: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  3. Jia Yuan Zhu: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  4. Xue Sen Fu: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  5. Jing Yu Zhang: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  6. Jia Wei He: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  7. Xiao Rong Liu: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  8. Zhi Hui Wang: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  9. Qiao Zhen Tong: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  10. Xiang Dan Liu: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
  11. Ri Bao Zhou: School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
PMID: 40104033 DOI: 10.3389/fpls.2025.1533263

Abstract

Background: Hand.-Mazz., the primary sources of Lonicerae Flos(Shanyinhua), brings great medicinal and economic value as an invaluable source of natural bioactive compounds. Nutrient and metabolites accumulation generally changed accompany with its floral development and opening. While the specific accumulation pattern and the underlying molecular regulatory networks remain unclear.
Methods: The present study intergrated a comparative analysis upon UPLC-MS/MS-based metabolomics and RNA-seq-based transcriptomics to revealed the differences in accumulation of flavonoids, phenolic acids, and terpenoids between the xianglei-type (corolla-closed) and wild-type (corolla-unfolded) of flowers.
Results and conclusion: 674 differentially accumulated metabolites(DAMs) were identified in WT and XL, with 5,776 common differentially expressed genes(DEGs), revealing a significant differences in accumulation of flavonoids, phenolic acids, and terpenoids during the late stage of flower development between the xianglei-type and wild-type of flowers. Combined analysis further identified 36 hub genes, major transcription factors and hormone-related genes, which play key roles in the differential accumulation of the abovementioned metabolites. These lines of evidences provide a molecular basis for the metabolic changes occurring during growth and can be significantly implicated in further research on the biosynthetic pathways associated with high-value potent active components in woody plants.

Keywords

Lonicera macranthoides Hand.-Mazz. active ingredients metabolome regulatory network transcriptome

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Created with Highcharts 10.0.0accumulationmetabolitesdifferencesgenesHand-Mazzdevelopmentmolecularregulatoryanalysisflavonoidsphenolicacidsterpenoidsxianglei-typewild-typeflowersdifferentiallyidentifiedhigh-valueactiveingredientsmetabolometranscriptomeBackground:primarysourcesLoniceraeFlosShanyinhuabringsgreatmedicinaleconomicvalueinvaluablesourcenaturalbioactivecompoundsNutrientgenerallychangedaccompanyfloralopeningspecificpatternunderlyingnetworksremainunclearMethods:presentstudyintergratedcomparativeuponUPLC-MS/MS-basedmetabolomicsRNA-seq-basedtranscriptomicsrevealedcorolla-closedcorolla-unfoldedResultsconclusion:674accumulatedDAMsWTXL5776commonexpressedDEGsrevealingsignificantlatestageflowerCombined36hubmajortranscriptionfactorshormone-relatedplaykeyrolesdifferentialabovementionedlinesevidencesprovidebasismetabolicchangesoccurringgrowthcansignificantlyimplicatedresearchbiosyntheticpathwaysassociatedpotentcomponentswoodyplantsAccumulationdifferentphenotypeinsightsintegrativeanalysesLoniceramacranthoidesnetwork

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