This project that aims at identifying candidate genes associated with cassava response to mealybugs (Phenacoccus manihoti) through transcriptome profiling of cassava genotypes infested with mealybugs.
A time-course transcriptome analysis of two cassava varieties that are either resistant or susceptible to cassava brown streak disease (CBSD) was conducted using RNASeq, after graft inoculation with Ugandan cassava brown streak virus (UCBSV).
β-carotene concentrations in cassava storage roots were enhanced by co-expression of transgenes for deoxyxylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin type-1 promoter. Transcriptom data was generated from cassava storage roots harvetsed at 12 months after planting from confined field trial conducted in Puerto Rico. Two transgenic cassava lines DXS//PS-20 and DXS//PS-37 and a non-tranegnic control cultivar 60444 were used for the study each replicated three-times.
dual RNA-seq experiment on fine roots of cassava (Manihot esculenta) colonized by the arbuscular mycorrhizal fungus Rhizophagus irregularis grown in the greenhouse. one plant- one fungi experiment. in total 5 different plant cultivars x 2 strains of fungi tested independently
We evaluated the plant and fungal genome wide RNA expression of the AM fungi Rhizophagus irregularis when colonizing cassava fine roots in a greenhouse experiment. The plants were grown in sterile soil and inoculated with Rhizophagus irregularis spores.