Introduction

SUMMARY: Analysis of differential gene expression by RNA sequencing (RNA-Seq) is frequently done using feature counts, i.e. the number of reads mapping to a gene. However, commonly used count algorithms (e.g. HTSeq) do not address the problem of reads aligning with multiple locations in the genome (multireads) or reads aligning with positions where two or more genes overlap (ambiguous reads). Rcount specifically addresses these issues. Furthermore, Rcount allows the user to assign priorities to certain feature types (e.g. higher priority for protein-coding genes compared to rRNA-coding genes) or to add flanking regions. AVAILABILITY AND IMPLEMENTATION: Rcount provides a fast and easy-to-use graphical user interface requiring no command line or programming skills. It is implemented in C++ using the SeqAn (www.seqan.de) and the Qt libraries (qt-project.org). Source code and 64 bit binaries for (Ubuntu) Linux, Windows (7) and MacOSX are released under the GPLv3 license and are freely available on github.com/MWSchmid/Rcount. CONTACT: marcschmid@gmx.ch SUPPLEMENTARY INFORMATION: Test data, genome annotation files, useful Python and R scripts and a step-by-step user guide (including run-time and memory usage tests) are available on github.com/MWSchmid/Rcount.

Publications

  1. Rcount: simple and flexible RNA-Seq read counting.
    Cite this
    Schmid MW, Grossniklaus U, 2015-02-01 - Bioinformatics (Oxford, England)

Credits

  1. Marc W Schmid
    Developer

    Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zurich, Switzerland

  2. Ueli Grossniklaus
    Investigator

    Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zurich, Switzerland

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Summary
AccessionBT000120
Tool TypeApplication
Category
PlatformsLinux/Unix
TechnologiesC++
User InterfaceTerminal Command Line
Download Count0
Country/RegionSwitzerland
Submitted ByUeli Grossniklaus